نتایج جستجو برای: 4 dinitrophenylhydrazine

تعداد نتایج: 1303745  

2016
Esin Akgul Kalkan Mehtap Sahiner Dilek Ulker Cakir Duygu Alpaslan Selehattin Yilmaz

Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood. The determination was carried out at 365 nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD). For acetone as its 2,4-dinitrophenylhydrazone derivative, a good sepa...

Journal: :The Journal of experimental biology 2011
Andrew Bradshaw Michael Salt Ashley Bell Matt Zeitler Noelle Litra Andrew M Smith

The terrestrial slug Arion subfuscus secretes a glue that is a dilute gel with remarkable adhesive and cohesive strength. The function of this glue depends on metals, raising the possibility that metal-catalyzed oxidation plays a role. The extent and time course of protein oxidation was measured by immunoblotting to detect the resulting carbonyl groups. Several proteins, particularly one with a...

Journal: :Environmental Health Perspectives 1996
T W Robison H Zhou K J Kim

Pulmonary injury from nitrogen dioxide (NO2) may in part be related to the generation of aldehydic compounds, which bind with cellular proteins and subsequently impair or inhibit cell function. We examined the generation of aldehydes from guinea pig tracheobronchial epithelial (GPTE) cell monolayers exposed to NO2. With the use of dinitrophenylhydrazine (DNP) to derivatize aldehydic compounds, ...

Arman Seri, Azadeh Hamedi Marjan Khorsand Mohammad Ali Takhshid, Zahra Rezaei,

Background: Glucose-induced protein glycation has been implicated in the progression of diabetic complications and age-related diseases. The anti-glycation potential of polyphenol-rich plant extracts has been shown previously. Bunium Persicum has been demonstrated to possess a high level of polyphenols. The aim of current in vitro study was to determine the possible inhibitory effect of Bunium ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1986
L Latriano B D Goldstein G Witz

It has been proposed that a ring-opened form may be responsible for the toxicity of benzene. The present studies demonstrate that incubation of [14C]benzene with liver microsomes (obtained from male CD-1 mice treated with benzene) in the presence of NADPH results in the formation of a ring-opened product. Evidence for the identity of this product was obtained by derivatizing with 2-thiobarbitur...

2003
ROBERT RIDGELY SEALOCK HANNAH E. SILBERSTEIN

In the course of the experiments demonstrating that the metabolism of the melanin pigment precursors, tyrosine and dihydroxyphenylalanine, increases the vitamin C requirement of the guinea pig (1) it was learned that those animals receiving tyrosine excreted homogentisic acid in the urine. Furthermore, the amount of the metabolite appeared to be inversely proportional to the amount of ascorbic ...

Journal: :Free radical biology & medicine 2012
Graziano Colombo Isabella Dalle-Donne Marica Orioli Daniela Giustarini Ranieri Rossi Marco Clerici Luca Regazzoni Giancarlo Aldini Aldo Milzani D Allan Butterfield Nicoletta Gagliano

Cigarette smoke, a complex mixture of over 7000 chemicals, contains many components capable of eliciting oxidative stress, which may induce smoking-related disorders, including oral cavity diseases. In this study, we investigated the effects of whole (mainstream) cigarette smoke on human gingival fibroblasts (HGFs). Cells were exposed to various puffs (0.5-12) of whole cigarette smoke and oxida...

Journal: :Journal of proteome research 2007
R Lemaire A Desmons J C Tabet R Day M Salzet I Fournier

Formalin fixation, generally followed by paraffin embedding, is the standard and well-established processing method employed by pathologist. This treatment conserves and stabilizes biopsy samples for years. Analysis of FFPE tissues from biopsy libraries has been, so far, a challenge for proteomics biomarker studies. Herein, we present two methods for the direct analysis of formalin-fixed, paraf...

Journal: :Frontiers in bioscience : a journal and virtual library 2001
S Adams P Green R Claxton S Simcox M V Williams K Walsh C Leeuwenburgh

The spectrophotometric protein carbonyl assay is used as an indicator of protein damage by free radical reactions in vitro and in a variety of pathologies. We investigated model proteins and a variety of oxidative and non-oxidative reactions, as well as what effects hemoglobin, myoglobin, and cytochrome c might have on levels of protein carbonyls. We show that oxidative as well as non-oxidative...

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