The study of inorganic crystalline materials by solid-state NMR spectroscopy is often complicated by the low sensitivity of heavy nuclei. However, these materials often contain or can be prepared with paramagnetic dopants without significantly affecting the structure of the crystalline host. Dynamic nuclear polarization (DNP) is generally capable of enhancing NMR signals by transferring the mag...

Highly water-soluble ubiquinone-0 (CoQ0) reacts with ascorbate monoanion (Asc) to mediate the production of ascorbyl free radicals (AFR). Using aqueous reaction mixture of CoQ0 and Asc, we obtained positively enhanced dynamic nuclear polarization (DNP)-magnetic resonance (MR) images of the AFR at low frequency (ranging from 515 to 530 MHz) of electron spin resonance (ESR) irradiation. The shape...

TR- mutant rats have an autosomal recessive mutation that is expressed as a severely impaired hepatobiliary secretion of organic anions like bilirubin-(di)glucuronide and dibromosulphthalein (DBSP). In this paper, the hepatobiliary transport of glutathione and a glutathione conjugate was studied in normal Wistar rats and TR- rats. It was shown that glutathione is virtually absent from the bile ...

Objective To assess the safety profile of lacosamide monotherapy in elderly (≥65 years) subjects with diabetic neuropathic pain (DNP). Methods Of 1,863 DNP subjects in double-blind, randomized, placebo-controlled trials of lacosamide monotherapy (NCT00861445, NCT00235469, NCT00238524, NCT00135109, NCT00350103), 502 were elderly. Safety data from elderly subjects were compared with that of you...

OBJECTIVE The mitochondrial uncoupling agent 2,4-dinitrophenol (DNP), historically used as a treatment for obesity, is known to cross the blood-brain-barrier, but its effects on central neural circuits controlling body weight are largely unknown. As hypothalamic melanocortin neuropeptide Y/agouti-related protein (NPY/AgRP) and pro-opiomelanocortin (POMC) neurons represent key central regulators...

Diabetic Nephropathy (DNP) is a chronic disease caused by diabetes that leads to end stage renal diseases. Although various pathological mechanisms have been proposed till date on the progression of DNP, the exact cause of this disease is still unknown. Here, we have focused on the modifications of low density lipoproteins (LDL) and its pathogenicity in DNP. LDL modification, specifically oxida...

The ability of guinea pigs to recognize poly-I,-lysine (PLL), and hapten conjugates of PLL (H-PLL) as antigens is under the control of a single autosomal dominant gene which we will refer to as the PLL gene (1-3). Responder animals carrying this gene form specific antihapten antibodies and display delayed hypersensitivity reactions after immunization with H-PLL conjugates such as 2,4-dinitrophe...

Dynamic Nuclear Polarization (DNP) enhances signal to noise in NMR experiments, by transferring the large electron Boltzmann polarization to nuclear polarization, via application of pulsed or continuous-wave microwave irradiation. This results in increases in NMR sensitivity of 2-3 orders of magnitude. DNP greatly reduces experimental times and makes some experiments possible that are otherwise...

Dynamic Nuclear Polarization (DNP) increases the sensitivity of NMR spectroscopy by using high frequency microwaves to transfer the polarization of the electrons to the nuclear spins. The enhancement in NMR sensitivity can amount to a factor of well above one hundred, enabling faster data acquisition and greatly improved NMR measurements. With the increasing magnetic fields (up to 23T) used in ...

The migration inhibitory factor (MIF) production of peripheral lymphocytes following exposure to dinitrophenylated microsomes derived from both human (DNP-hy-Mic) and guinea pig (DNP-gp-Mic) epidermis was quantitated to detect human contact sensitivity to dinitrochlorobenzene (DNCB). The lymphocytes from nonsensitized subjects did not generate MIF following exposure to either antigen. With DNP-...