1. A constant molecular weight of 57000 was obtained by gel filtration of highly purified acetyl-CoA synthetase over a 1000-fold range of enzyme concentrations. The amino acid analysis is reported. 2. With native enzyme at 20 degrees C the relatively rapid reaction of four thiol residues with p-hydroxymercuribenzoate caused an immediate inhibition reversible by either CoA or mercaptoethanol. Ot...

This study highlights the utilization of Cr2S3-Cr2O3/P2ABT nanocomposite photoelectrode for efficient and highly sensitive photon absorption, enabling generation green hydrogen through production hot electrons upon illumination. The is synthesized via a one-pot reaction using K2Cr2O7 2-aminobenzene-1-thiol monomer, presence Cr2S3-Cr2O3 confirmed by XRD XPS analysis within composite. optical pro...

we give the concept of ‘braiding’ for 2-groupoids, and we show that this structure is equivalent tobraided regular, crossed modules.

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A free sulfhydryl group previously has been shown to be required for carbohydrate binding to the lectin from lima bean (Phaseolus lunatus) (Gould, N. R. and Scheinberg, S. L. (1970) Arch. Biochem. Biophys. 141, 607-613). Modification of this group by sulfhydryl reagents was specifically inhibited by D-GalNAc. We have further examined the reactivity of sulfhydryl groups in lima bean lectin with ...

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts with aliphatic thiols. A new thiolation procedure for proteins is based on this reagent. The procedure involves two steps. First, 2-pyridyl disulphide structures are introduced into the protein by...

The intracellular redox state is stringently maintained by thiol-based antioxidants to establish a balance for the physiological and pathophysiological roles of reactive oxygen species. The relative contributions of the thioredoxin (Trx) and glutathione/glutaredoxin systems to intracellular redox balance are incompletely understood, as are the consequences of altered thiol metabolism on endothe...

Hybrid molecular brushes (HMBs), or bottlebrush polymers, are polymeric constructs containing a linear backbone and side chains with different chemical characteristics. Hybrid-block graft copolymers (HCPs) similar to traditional HMBs, except their consists of more than one polymer. We developed facile method for the synthesis family amphiphilic, biodegradable HCPs composed two blocks: hydrophil...

Human tryptophanyl-tRNA synthetase resembles its counterpart in Escherichia coli in quaternary structure (alpha2), but differs in molecular weight, amino acid composition, the number of thiol groups, and the relationship of the thiol groups to enzyme activity. Nevertheless, one of the thiol groups resides in a heptapeptide sequence homologous to a heptapeptide sequence containing a thiol group ...

BACKGROUND  β(2) -Glycoprotein I (β(2) GPI) is an abundant plasma protein that is closely linked to blood clotting, as it interacts with various protein and cellular components of the coagulation system. However, the role of β(2) GPI in thrombus formation is unknown. We have recently shown that β(2) GPI is susceptible to reduction by the thiol oxidoreductases thioredoxin-1 and protein disulfide...