The COQ2 gene in Saccharomyces cerevisiae encodes a Coq2 (p-hydroxybenzoate:polyprenyl transferase), which is required in the biosynthetic pathway of CoQ (ubiquinone). This enzyme catalyses the prenylation of p-hydroxybenzoate with an all-trans polyprenyl group. We have isolated cDNA which we believe encodes the human homologue of COQ2 from a human muscle and liver cDNA library. The clone conta...

/3-c-p region with a cross-over bust be that of phydroxybenzoate hydroxylase. The only conventional nucleotide-binding site is occupied by FAD, and NADP+ binds to the enzyme together with FAD for turnover. It may be significant that for the two observed variant coenzyme sites, those of hexokinase and 6-phosphogluconate dehydrogenase, the details of coenzyme binding are said to be ‘different’ (S...

The dissimilatory Fe(III) reducer, GS-15, is the first microorganism known to couple the oxidation of aromatic compounds to the reduction of Fe(III) and the first example of a pure culture of any kind known to anaerobically oxidize an aromatic hydrocarbon, toluene. In this study, the metabolism of toluene, phenol, and p-cresol by GS-15 was investigated in more detail. GS-15 grew in an anaerobic...

In Streptomyces coelicolor, we identified a para-hydroxybenzoate (PHB) hydroxylase, encoded by gene pobA (SCO3084), which is responsible for conversion of PHB into PCA (protocatechuic acid), a substrate of the β-ketoadipate pathway which yields intermediates of the Krebs cycle. We also found that the transcription of pobA is induced by PHB and is negatively regulated by the product of SCO3209, ...

Toluene and m-xylene were rapidly mineralized in an anaerobic laboratory aquifer column operated under continuous-flow conditions with nitrate as an electron acceptor. The oxidation of toluene and m-xylene was coupled with the reduction of nitrate, and mineralization was confirmed by trapping 14CO2 evolved from 14C-ring-labeled substrates. Substrate degradation also took place when nitrous oxid...

Acinetobacter PobR and PcaU are transcriptional activators that closely resemble each other in primary structure, DNA-binding sites, metabolic modulators, and physiological function. PobR responds to the inducer-metabolite p-hydroxybenzoate and activates transcription of pobA, the structural gene for the enzyme that converts p-hydroxybenzoate to protocatechuate. This compound, differing from p-...

By site-directed mutagenesis, we have changed Asn300 to Asp in p-hydroxybenzoate hydroxylase (PHBH; EC 1.14.13.2) from Pseudomonas aeruginosa. In the wild-type (WT) enzyme, residue 300 is in contact with the isoalloxazine ring of the active-site FAD; in the Asn300Asp mutant, this side chain has moved by about 5 A, altering the protein structure [Lah, M.S., Palfey, B.A., Schreuder, H.A., & Ludwi...

The pca branch of the 0-ketoadipate pathway in Pseudomonas putida is responsible for the complete degradation ofp-hydroxybenzoate through ortho cleavage of the initial pathway metabolite, protocatechuate. The pcaR regulatory locus has been found to be required for both induction of all of the genes within the pca regulon (pcaBDC, pcaUj, and peaF) and the chemotactic response of the bacteria to ...

Repression of enzymes contributing to degradation of aromatic compounds via the beta-ketoadipate pathway in the presence of additional carbon sources (carbon catabolite repression) in the bacterium Acinetobacter sp. strain ADP1 is described. The phenomenon was investigated on the level of specific activity of protocatechuate 3,4-dioxygenase and p-hydroxybenzoate hydroxylase participating in cat...

in this paper, we introduce the concepts of $2$-isometry, collinearity, $2$%-lipschitz mapping in $2$-fuzzy $2$-normed linear spaces. also, we give anew generalization of the mazur-ulam theorem when $x$ is a $2$-fuzzy $2$%-normed linear space or $im (x)$ is a fuzzy $2$-normed linear space, thatis, the mazur-ulam theorem holds, when the $2$-isometry mapped to a $2$%-fuzzy $2$-normed linear space...