BACKGROUND Recent advances in cell therapies have provided potential opportunities for the treatment of chronic kidney diseases (CKDs). We investigated whether human kidney structures could be preformed in vitro for subsequent implantation in vivo to maximize tissue-forming efficiency. METHODS Human renal cells were isolated from unused donor kidneys. Human renal cells were cultured and expan...

In vivo cells form three-dimensional (3D) microenvironments through cell-cell and cell-matrix interactions to create complex tissues and perform higher level functions. Biochemical signals and nutrient gradients are essential for tissue functioning and therefore also physiological cell behavior. Conventional two-dimensional cell culture does not provide such a complex microenvironment and conse...

There has been tremendous interest in constructing in vitro cardiac tissue for a range of fundamental studies of cardiac development and disease and as a commercial system to evaluate therapeutic drug discovery prioritization and toxicity. Although there has been progress towards studying 2-dimensional cardiac function in vitro, there remain challenging obstacles to generate rapid and efficient...

background: this study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (lh) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. materials and methods: monolayers for co-culture were prepared from ovine oviduct epithelial cells (ooec) in dmem/f12 medi...

Three-dimensional (3D) printing has evolved massively during the last years and is demonstrating its potential in tissue engineering, wound dressings, cell culture models for drug testing, prosthesis, to name a few [...]

background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...

due to the number of new substances coming into use every year and the increasing amounts of chemicals, which are introduced into the environment, there is a high demand for a rapid, reliable and cost-effective method for detection of developmental toxicity. to meet this challenge various in vitro techniques have been established additional to in vivo animal testing. this review introduces the ...

objective: mycoplasma salivarium (m. salivarium) isone of the most common contaminants present in cell culture laboratories that cause undesirable effects on cell cultures. thus, the identification and rapid diagnosis in controlling and prevention of this contaminant are important. the aim of this study is the detection of mycoplasma salivarium contamination in cell culture using polymerase cha...

background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...