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Several methods have been described for gene walking by PCR on uncloned DNA (2 — 13). In general, they suffer from limitations: they either avoid high-complexity whole genomes, require delicate manipulation or give contaminated products. This report describes a reliable and simple PCR (1) adaptation for gene walks from any specific genomic locus: unpredictably primed PCR (UP-PCR). Besides its s...
in [u. dempwolff, on extensions of elementary abelian groups of order $2^{5}$ by $gl(5,2)$, textit{rend. sem. mat. univ. padova}, textbf{48} (1972), 359 - 364.] dempwolff proved the existence of a group of the form $2^{5}{^{cdot}}gl(5,2)$ (a non split extension of the elementary abelian group $2^{5}$ by the general linear group $gl(5,2)$). this group is the second l...
This study explored the concepts of intercultural competence and cultural intelligence and clarified corresponding terms. Using the Cultural Intelligence Scale (CQS) as an instrument and conducting multiple regression tests, this quantitative research investigated individual scale factors that would influence the intercultural competence of the students who declared an education major or minor ...
The structure of a modified uridine derivative which was detected at the first letter position of the anticodon of Bacillus subtilis tRNA1Lys was determined to be 5-(carboxymethylaminomethyl)-2-thiouridine. The determination was mainly based in this ultraviolet absorption spectra and mass spectrometric analysis of the trimethylsilyl derivative.
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