Background and Purpose—It has been reported that estrogens modulate peripheral vascular synthesis of vasodilatory hormones, including prostacyclin. If this occurs in the cerebral circulation, it could have important consequences in the modulation of cerebral hemodynamic function and improvement of stroke outcome. We investigated the hypothesis that in vivo 17 -estradiol treatment of ovariectomi...

Characterization of estrogen-binding components was attempted in cytosol fractions from thymus, spleen and mesenteric lymph node of castrated rats. As shown by sucrose gradient analysis, specific binding of [6,7-3H]estradiol-17 beta in the thymus is associated with a component migrating at 4 S. The binding of [6,7-3H[estradiol-17 beta is highly specific since it is easily displaced by unlabeled...

We previously reported that 17 beta-estradiol inhibits cytokine-stimulated bioassayable IL-6 and the steady-state level of IL-6 mRNA. To determine the molecular basis of this effect, the transient expression of chloramphenicol acetyltransferase (CAT) reporter plasmid driven by the human IL-6 promoter was studied here in HeLa or murine bone marrow stromal cells (MBA 13.2). 17 beta-estradiol (10(...

(ES) by hepatic microsomes from male rats, we found the formation of two metabolites, and identified them as 2and 4hydroxyestradiol 17-sulfates (2and 4-OH-ES, Fig. 1) by comparisons with authentic specimens.1 In female rats, in contrast, at least three metabolites in addition to the abovementioned two catechol metabolites were detected during HPLC.1 The identification of structure-unknown metab...

A time-resolved fluoroimmunoassay (TR-FIA) for unconjugated estrogens in human urine is described. 6-Keto-17 beta-estradiol-6-(O-carboxymethyl)oxime:bovine serum albumin is immobilized onto microtiter strip wells and the coated wells are incubated with 17 beta-estradiol standard preparations or unknowns with a polyclonal antiserum to 17 beta-estradiol-16,17-monosuccinyl:albumin. The antiserum-b...

A single transient dose of estradiol-17 beta is sufficient to elicit the permanent induction of hepatic estrogen receptor mRNA, which is induced 18-fold (from 0.13 to 2.4 molecules per cell) and then remains fully induced for at least 125 days. In primary liver cultures, extremely low concentrations of estradiol-17 beta, which are below the Kd of the Xenopus laevis estrogen receptor, maintain p...

One minute after instillation of 14C-estradiol-17 beta (14C-E2 17 beta) into selected sections of the gastrointestinal tract of swine, radioactive estradiol metabolites were present in blood collected from the portal and jugular veins. Ether was used to extract free but not conjugated estrogens. The percentage of plasma radioactivity that was ether extractable (EE) was low in portal plasma and ...

Mammary carcinoma induced in male Sprague-Dawley rats by multiple intragastric intubations of 7,12-dimethylbenz-(a)anthracene showed ovary-independent growth but contained estrogen receptors (ER) and progesterone receptors (Yoshida, Yoshida. Fukunishi, Sato, Okamoto, and Matsumoto, Cancer Res., 42: 2434-2439, 1982). Transplantable carcinoma (MT6) was obtained from dimethylbenz(a)anthracene-indu...

Although estrogenic activity of ovarian extracts has been reported for all classes of vertebrates and certain invertebrates, the chemical nature of the active compounds has been identified only in mammals. The hormone of the graafian follicle was isolated by MacCorquodale, Thayer, and Doisy (1) from liquor folliculi of sow ovaries and named dihydrotheelin, a synonym of estradiol-17p according t...

A soluble enzyme from the small intestine of the pig, capable of conjugating uridine diphosphate glucuronic acid with 17/3-estradiol, has been purified 5to IO-fold. The glucuronide formed was identified as 17P-estradiol J-monoglucuronide by paper chromatography and microchemical reactions. This estrogen glucuronyltransferase is present in the 150,000 x g supernatant and shows an absolute requir...