We have studied Hansenula polymorpha Pex5p and Pex20p, peroxins involved in peroxisomal matrix protein import. In vitro binding experiments suggested that H. polymorpha Pex5p and Pex20p physically interact. We used single particle electron microscopy (EM) to analyze the structure of purified Pex5p and its possible association with Pex20p. Upon addition of Pex20p, a multimeric Pex20p complex was...

The use of refrigerant mixtures in refrigeration machines has been proposed in the technical literature. Some but not all of the arguments for and against the use of mixtures as refrigerants are examined in this study. An issue associated with use of blends is the segregation on leakage and the ensuing loss in capacity and performance change on repeated recharging with the original mixture. We ...

This paper presents the numerical analysis of a Miniature-Scale Refrigeration System (MSRS) for electronics cooling. The system consists of a simulated electronic chip attached to a microchannel cold plate evaporator, a compressor, a microchannel condenser, and an expansion device. The system uses R-134a as the refrigerant. A copper block heater is designed to simulate the heat generation of an...

In many cases, polyol ester (POE) and polyalkylene glycol (PAG) lubricants used with HFC refrigerants, may cause long-term reliability and stability problems. Mineral oil lubricants are much more stable in the long run and do not suffer from stability problems nearly as much as POE and PAG lubricants. HFC based refrigerants and blends are, in general, nonmiscible in mineral oils, and will not r...