Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

Molecular characterization of eleven clones of C. winterianus collected from eleven districts of West Bengal was carried out with 10 RAPD and 9 ISSR primers. The RAPD primers developed 81 robust loci, which revealed 74.07% polymorphism and 61 ISSR markers generated 47.50% polymorphism. Out of the different anchored primer combinations, ISSR primers with AG and GA motifs produced clear and maxim...

The pomegranate is one of the oldest fruits that are traditionally consumed by the local inhabitants of the Coruh Valley, Turkey. In this study, the molecular and morphological characteristics of 19 promising pomegranate genotypes selected from the Coruh Valley were evaluated. For the morphological evaluation, 22 quantitative fruit characteristics were used. For the molecular evaluation, 47 ran...

This study evaluated the optimal vegetative growth conditions and molecular phylogenetic relationships of eleven strains of Agrocybe cylindracea collected from different ecological regions of Korea, China and Taiwan. The optimal temperature and pH for mycelial growth were observed at 25℃ and 6. Potato dextrose agar and Hennerberg were the favorable media for vegetative growth, whereas glucose t...

Random genetic changes generated during in vitro culture are not desirable for plant micropropagation and genetic transformation. RAPD markers were used to detect the variation in leaf disc callus cultures of Jatropha curcas, maintained in Murashige and Skoog (MS) medium with different auxin and cytokinin combinations. In total 41 scorable bands were produced with 11 primers. Out of 41 bands, 3...

almond is one of the most valuable horticultural nut crops in the world with iran being amongst the most important regions of its distribution. rapd markers were employed to evaluate the diversity levels and relationship among some 56 collected genotypes of the crop. through sixteen rapd primers a number of, 171 dna bands were produced, 168 of which were polymorphic. the samples which according...

The randomly amplified polymorphic DNA (RAPD) technique was used in the development of a fingerprinting (typing) and identification protocol for Legionella strains. Twenty decamer random oligonucleotide primers were screened for their discriminatory abilities. Two candidate primers were selected. By using a combination of these primers, RAPD analysis allowed for the differentiation between all ...

In this paper, nine geographical isolates of Schistosoma mansoni (three from Egypt, three from Saudi Arabia and 3 from Puerto Rico) were studied at the genotype level by RAPD analysis with two arbitrary primers. The genetic distance was measured by the percentage of unshared bands. The RAPD results showed that the Egyptian strains were closely related to Saudi strains but Puerto Rico strains cl...

Canna indica Linn. (Cannaceae) is used both as medicine and food. Traditionally, various parts of C. indica are exploited to treat blood pressure, dropsy, fever, inflammatory diseases etc. However, to date there is no reliable micropropagation protocol for C. indica. We present here a regeneration technique C. indica with banana micropropagation medium (BM). BM supplemented with 3% sucrose, 0.7...

Thirty-one species of Mammillaria were selected to study the molecular phylogeny using random amplified polymorphic DNA (RAPD) markers. High amount of mucilage (gelling polysaccharides) present in Mammillaria was a major obstacle in isolating good quality genomic DNA. The CTAB (cetyl trimethyl ammonium bromide) method was modified to obtain good quality genomic DNA. Twenty-two random decamer pr...