In this study, the effect of different DNA extraction procedures and primer sets on pyrosequencing results regarding the composition of bacterial communities in the ileum of piglets was investigated. Ileal chyme from piglets fed a diet containing different amounts of zinc oxide was used to evaluate a pyrosequencing study with barcoded 16S rRNA PCR products. Two DNA extraction methods (bead beat...

DNA polymerase lambda (Pol lambda) is one of several DNA polymerases suggested to participate in base excision repair (BER), in repair of broken DNA ends and in translesion synthesis. It has been proposed that the nature of the DNA intermediates partly determines which polymerase is used for a particular repair reaction. To test this hypothesis, here we examine the ability of human Pol lambda t...

The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer u...

AIMS Despite their ability to cause septic shock and myocardial dysfunction, components of Gram-negative bacterial cell walls, like lipopolysaccharide, have been shown in numerous studies to induce myocardial protection during ischaemia-reperfusion injury. Muramyl dipeptide (MDP) is another such component recognized by an intracellular receptor, nucleotide-binding oligomerization domain 2. Rece...

Objective: The purpose of this study was to evaluate the effect MDP containing resin cement and surface treatment Translucent zirconia Ultra translucent on shear bond strength. Material & Methods: Eighty discs were constructed . Samples divided into two groups: Group I: samples (n=20) Gro up II: Each group will be subdivided 2 subgroups according Subgroup A: treated with air abrasion Sub B: lef...

The self-etching primer adhesives examined in the present study were aqueous mixtures of 5 wt% MDP, 35 wt% HEMA, 3 wt% 2-hydroxyl-3-(3,4-dimethyl-9-oxo-9H-thioxanthen-2-yloxy)-N,N,N-trimethyl-1-propanaminium chloride (QTX), 0.5 wt% ethyl-p-(N,N-dimethylamino)benzoate (EDM), and 30 wt% water-soluble dimethacrylate (i.e., 2,2-bis(4-methacryloxy polyethoxypheny)propane (BPE), Glycerol dimethacryla...

Incidental extra-osseous uptake of 99mTc-MDP on bone scintigraphy has been frequently reported in the literature; however, calcification of the psoas muscle is a rare condition, which has been reported on magnetic resonance (MR) and computed tomography (CT) imagings. Here we report a 67-year-old woman with acute paraplegia and low back pain who was referred for a 99mTc-MDP...

N-trimethyl chitosan (TMC) nanoparticles have been shown to increase the immunogenicity of subunit antigens after nasal and intradermal administration. This work describes a second generation of TMC nanoparticles containing ovalbumin as a model antigen (TMC/OVA nanoparticles) and an immunopotentiator (TMC/OVA/immunopotentiator nanoparticles). The selection of immunopotentiators included Toll-li...