Primordial germ cells (PGCs) form at the posterior pole of the Drosophila embryo, and then migrate to their final destination in the gonad where they will produce eggs or sperm. Studies of the different stages in this process, including assembly of germ plasm in the oocyte during oogenesis, specification of a subset of syncytial embryonic nuclei as PGCs, and migration, have been informed by gen...

Hans Hansson, Christer Norstr om, and Sasikumar Punnekkat M alardalen Real-Time Resear h Centre Department of Computer Engineering Malardalen University, Vaster as, SWEDEN han idt.mdh.se, en mdh.se, spt idt.mdh.se WWW home page: http://www.mrt .mdh.se Abstra t. In traditional design of omputer based systems some e ort, say 1, is spent on the early modeling phases, and some very high e ort, ...

Previous studies have demonstrated that verbal descriptions of actions activate compatible motor responses (Glenberg & Kaschak, 2002; Zwaan & Taylor, 2006). The present study replicates previous findings showing that, within a sentence, such activation is localized on the verb that denotes the action. Moreover, motor resonance is found to yield to linguistic focus. If a postverbal adverb mainta...

There are several publications describing the SC quad design for TESLA [2] and for ILC [3] which could be useful for implementation in ERL. Design of [3] repeats earlier design described in [2]. However neither of these publications contains the field throughthe-lens-integrals as function of transverse offset. Meanwhile 3D field behavior is crucial for the low emittance beams, which are ILC and...

1 The Rockefeller University Press J. Cell Biol. https://doi.org/10.1083/jcb.201705017 Introduction The plasma membrane (PM) serves as a physical barrier that separates the cytosolic milieu of the cell from the comparatively harsh external chemical environment. It also serves as a sophisticated communication platform through which cells receive and respond to messages from each other, as well a...

The well-known regenerative abilities of planarian flatworms are attributed to a population of adult stem cells called neoblasts that proliferate and differentiate to produce all cell types. A characteristic feature of neoblasts is the presence of large cytoplasmic ribonucleoprotein granules named chromatoid bodies, the function of which has remained largely elusive. This study shows that histo...

RNase P is an RNA-based enzyme primarily responsible for 50-end pre-tRNA processing. A structure of the bacterial RNase P holoenzyme in complex with tRNA revealed the structural basis for substrate recognition, identified the active site location, and showed how the protein component increases functionality. The active site includes at least two metal ions, a universal uridine (U52), and P RNA ...

Hedgehog signaling is crucial for vertebrate development and physiology. Gli2, the primary effector of Hedgehog signaling, localizes to the tip of the primary cilium, but the importance of its ciliary localization remains unclear. We address the roles of Gli2 ciliary localization by replacing endogenous Gli2 with Gli2, a Gli2 variant not localizing to the cilium. The resultingGli2 andGli2;Gli3 ...

Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent accumulation of undesired off-target mutations. Here, we present a novel strate...