Tissue culture techniques are widely used for the mass propagation of many species. In cashew in vitro propagation, some protocols need to be established at this end. The present work was carried out evaluate conditions regeneration seedlings from micropropagation by organogenesis on Benin genotypes. Nodal explants one-month-old greenhouse and cotyledonary nodes germination were purpose. BAP ki...

gerbera (gerbereajamesonii) cut flower has magnificent economical value in international cut flower industry. due to sensitivityto ethylene and to gray mold, it has a short postharvest life. in this research, the effects of pulsing treatments with salicylic acid (100 and 200 mg/l) and methyl jasmonate (25 and 50 mg/l) for 24h on quality and vase-life of gerbera cut flower were assessed. distill...

Cycad is one of the most important cut foliage plants with several usages. This experiment was focused on increasing the vase life of Cycad’s cut foliage in normal storage condition. Experiment was based on a CRD with three replications. Treatments were conducted for four different levels of gibberellic acid (GA3) and benzyl adenine (BA) each one with 0, 50, 100 and 150 mg L-1, according to men...

I describe the use of latex agglutination as a simple readout for DNA hybridization assays. Latex agglutina-tion is a well-known readout for antibody-based diagnos-tics (1, 2) and uses " sandwich " assay chemistry for which analogous DNA assays are well known (3, 4). Latex agglutination is extremely simple and chemically robust, and latex particles have been used as a label in DNA hybridization...

the aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºc for 0, 12, 24, 48 and 72 hrs after collection anddilution. a total of 57 ejaculates from 12 bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

A 3 × 2 factorial experiment was conducted to investigate the effect of thymol + carvacrol (0, 100 and 200 mg/kg) on ileal microbial population and jejuna and ileal histomorphology of broilers fed Carboxy Methyl Cellulose (CMC: 0 and 2%) containing diet. Each of the 6 dietary treatments was replicated 5 times with 12 chicks each from 0 to 42 days of age. There was no interaction effect of CMC a...

Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequenc...

An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige Skoog media supplemented with 3% sucrose, 0.8% agar, various concentrations indole-3-butyric acid (1 to 10 mg/L). Cultures maintained 4 5 weeks at standard conditions allow root induction elongation. Shoot triggered upon...

a very simple, rapid and sensitive dispersive liquid-liquid microextraction (dllme) followed by gas chromatography and flame ionization detection (gc-fid) was developed for the determination of phenol and carvacrol in honey samples. a mixture of 100 µl dichloromethane (extraction solvent) and 0.5 ml acetonitrile (disperser solvent) was rapidly injected into sample solution. thereby a cloudy sol...