-Galactosidase is widely used in food industry to improve sweetness, solubility, flavor and digestibility of dairy products (Richmond, Gray, and Stine, 1981; Grosova et al. 2008a) . Enzymatic hydrolysis of lactose by galactosidase is one of the most popular technologies to produce lactose reduced milk and related dairy products for consumption by lactose intolerant people (Ladero, Santos, and G...

The effects of lactose, galactose, glucose, and glucose combined with galactose on the induction of β–galactosidase by IPTG in Escherichia coli strain VC10, growing in a glycerol media, were compared against a control of glycerol plus IPTG. After inducing β– galactosidase production with the IPTG, the cultures were altered with the addition of one of the above carbohydrates or combinations. Thi...

The lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, encoding a β-galactosidase of the glycoside hydrolase family GH2, was cloned into different inducible lactobacillal expression vectors for overexpression in the host strain Lactobacillus plantarum WCFS1. High expression levels were obtained in laboratory cultivations with yields of approximately 53000 U of β-galactosidase...

The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. β-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster stai...

α-Galactosidase from Penicillium purpurogenum was induced to a greater extent in the culture medium by arabinose, galactose, lactose and guar galactomannan. The enzyme was purified by acetone precipitation and DEAE-Sephacel column chromatography. The purified enzyme showed a single band in both native-PAGE and SDS-PAGE. The molecular mass of enzyme was found to be between 107 and 110 kDa. The e...

It has been shown that the insertion of antigenic peptides of the foot-and-mouse disease virus (FMDV) into specific sites of the bacterial enzyme β-galactosidase causes a severe reduction of the activity of this enzyme which can be recovered upon antibody binding to the inserted antigenic peptide [1]. In principle, the recovery of β-galactosidase activity upon antigen-antibody binding allows th...

a-Galactosidase production by a novel actinomycete strain Streptomyces griseoloalbus in shake flask culture was optimized using response surface methodology. Screening of variables to find their relative effect on a-galactosidase production was done using Plackett-Burman design. Out of the eleven factors screened, salinity, magnesium sulphate and temperature were found to influence the enzyme p...

An improved method is described for the preparation of bovine testicular beta-galactosidase that allows the isolation of enzyme fractions that bind avidly to phosphomannosyl receptors. The procedure permits removal of a contaminating beta-hexosaminidase and yields nearly homogeneous beta-galactosidase. Enzyme eluted from DEAE-Sephacel was arbitrarily divided into pools that exhibited differing ...

  Anderson-Fabry disease is a rare inherited X-linked lysosomal storage disease caused by deficiency of the enzyme alpha-galactosidase A. Hereby we report a 39 year old male that presented with proteinuria and edema. Histopathologic, immunofluorescence and ultrastractural examination of renal tissue were in favor  of  Fabry disease in associate with IgA nephropathy. Fabry's disease associated ...

The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, res...