A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetect...

A part of apples destined to juice production is generally of poor quality. Apples from cold storage or recently harvest (ground harvested or low quality apples) are stored under ambient conditions until they are processed. Since Penicillium expansum and P. griseofulvum are the principal fungal species isolated from stored apples in Brazil, the objective of this study was to investigate the abi...

Biocontrol activity of Metschnikowia pulcherrima alone and in combination with silicon against Penicillium expansum and its machanisms of antagonisms was evaluated. Percentage of inhibition mycelial growth of pathogen in dual culture, volatile test and non-volatile test were evaluated. To evaluate the direct effect of silicon on mycelial growth of P. expansum in vitro, it was added at different...

Penicillium spp. are known to harbor a wide array of secondary metabolites with cryptic bioactivities. However, the metabolomics of these species is not well-understood in terms of different fermentation models and conditions. The present study involved metabolomics profiling and transcriptomic analysis of Penicillium expansum 40815 under solid-state fermentation (SSF) and submerged fermentatio...

The biocontrol activity of Aureobasidium pullulans on decay of apple fruit caused by Botrytis cinerea and Penicillium expansum, and its ability to induce biochemical defense responses in apple tissue, were investigated. In apple wounds, A. pullulans multiplied rapidly and controlled decay caused by either B. cinerea or P. expansum. At the end of the storage period, A. pullulans reduced the inci...

Penicillium expansum Link causes an economically important postharvest blue mold disease in a number of fruits and vegetables. In the present study, this fungus was isolated from rotted grapes identified on morphological basis. Identification pathogen further confirmed molecular basis by using four different primer pairs namely ITS, β-tubulin, CMD CF under accession numbers MN752155, MN787831, ...

Apple fruits are susceptible to pathogenic fungi such as Botrytis cinerea and Penicillium expansum which responsible for significant pre- postharvest fruit losses. Given the strong restriction in use of synthetic agrochemicals, especially during phase, alternatives currently sought a more sustainable management apple storage. The antifungal activity thyme, clove, anise, camphor, juniper essenti...

The effect of ecological aspects of distribution of potential toxin-producing micromycetes on stored apple fruit was investigated at the Lithuanian Institute of Horticulture and Institute of Botany in 2004–2005. Fungi of twenty species belonging to eight genera were isolated from rotten and healthy apple fruits. Penicillium expansum and Penicillium italicum showed a high frequency of occurrence...

The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY...