نتایج جستجو برای: ژن xrcc4

تعداد نتایج: 16150  

2014
Pierre Hentges Helen Waller Clara C. Reis Miguel Godinho Ferreira Aidan J. Doherty

Eukaryotic cells use two principal mechanisms for repairing DNA double-strand breaks (DSBs): homologous recombination (HR) and nonhomologous end-joining (NHEJ). DSB repair pathway choice is strongly regulated during the cell cycle. Cyclin-dependent kinase 1 (Cdk1) activates HR by phosphorylation of key recombination factors. However, a mechanism for regulating the NHEJ pathway has not been esta...

Journal: :Blood 2016
Hanna IJspeert Jacob Rozmus Klaus Schwarz René L Warren David van Zessen Robert A Holt Ingrid Pico-Knijnenburg Erik Simons Isabel Jerchel Angela Wawer Myriam Lorenz Turkan Patıroğlu Himmet Haluk Akar Ricardo Leite Nicole S Verkaik Andrew P Stubbs Dik C van Gent Jacques J M van Dongen Mirjam van der Burg

Repair of DNA double-strand breaks (DSBs) by the nonhomologous end-joining pathway (NHEJ) is important not only for repair of spontaneous breaks but also for breaks induced in developing lymphocytes during V(D)J (variable [V], diversity [D], and joining [J] genes) recombination of their antigen receptor loci to create a diverse repertoire. Mutations in the NHEJ factor XLF result in extreme sens...

2015
Li Xu Hongwei Tang Adel K. El-Naggar Peng Wei Erich M. Sturgis Sue Cotterill

DNA double strand break (DSB) repair is the primary defense mechanism against ionizing radiation-induced DNA damage. Ionizing radiation is the only established risk factor for salivary gland carcinoma (SGC). We hypothesized that genetic variants in DSB repair genes contribute to individual variation in susceptibility to SGC. To test this hypothesis, we conducted a case-control study in which we...

2016
Michal Hammel Yaping Yu Sarvan K Radhakrishnan Chirayu Chokshi Miaw-Sheue Tsai Yoshihiro Matsumoto Monica Kuzdovich Soumya G Remesh Shujuan Fang Alan E Tomkinson Susan P Lees-Miller John A Tainer

DNA double-strand break (DSB) repair by non-homologous end joining (NHEJ) in human cells is initiated by Ku heterodimer binding to a DSB, followed by recruitment of core NHEJ factors including DNA-dependent protein kinase catalytic subunit (DNA-PKcs), XRCC4-like factor (XLF), and XRCC4 (X4)-DNA ligase IV (L4). Ku also interacts with accessory factors such as aprataxin and polynucleotide kinase/...

Journal: :Biochemistry 2010
Jeremiah Frye Vadim A Klenchin Ivan Rayment

Tropomyosin is a stereotypical alpha-helical coiled coil that polymerizes to form a filamentous macromolecular assembly that lies on the surface of F-actin. The interaction between the C-terminal and N-terminal segments on adjacent molecules is known as the overlap region. We report here two X-ray structures of the chicken smooth muscle tropomyosin overlap complex. A novel approach was used to ...

2010
Nicole Bennardo Jeremy M. Stark

Chromosome rearrangements can form when incorrect ends are matched during end joining (EJ) repair of multiple chromosomal double-strand breaks (DSBs). We tested whether the ATM kinase limits chromosome rearrangements via suppressing incorrect end utilization during EJ repair of multiple DSBs. For this, we developed a system for monitoring EJ of two tandem DSBs that can be repaired using correct...

Journal: :Biochemistry 2002
Dennis Merkle Pauline Douglas Greg B G Moorhead Zoya Leonenko Yaping Yu David Cramb David P Bazett-Jones Susan P Lees-Miller

DNA double-strand breaks are a serious threat to genome stability and cell viability. One of the major pathways for the repair of DNA double-strand breaks in human cells is nonhomologous end-joining. Biochemical and genetic studies have shown that the DNA-dependent protein kinase (DNA-PK), XRCC4, DNA ligase IV, and Artemis are essential components of the nonhomologous end-joining pathway. DNA-P...

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