نتایج جستجو برای: ژن gfp
تعداد نتایج: 31710 فیلتر نتایج به سال:
Green fluorescent protein (GFP) is a 27 kD protein consisting of 238 amino acid residues [1]. GFP was first identified in the aquatic jellyfish Aequorea victoria by Osamu Shimomura et al. in 1961 while studying aequorin, a Ca2+-activated photoprotein.Aequorin and GFP are local‐ ized in the light organs of A. victoria and GFP was accidentally discovered when the energy of the blue light emitted ...
قارچ ها در طبیعت به دلیل تولید آنزیم ها و متابولیت های اولیه و ثانویه ی بسیار مهم شان از موجودات بسیار مهم و پرکاربرد در صنعت، پزشکی و کشاورزی هستند. قارچ ها با روش های مختلف بیوتکنولوژیکی تحت دستورزی ژنتیکی قرار گرفته اند. بهترین روش توصیه شده برای دستورزی ژنتیکی قارچ ها روش انتقال ژن به واسطه آگروباکتریوم تومیفشینس شناخته شده است. قارچ کولتوتریکوم گلوسپوریویدس قارچی ملانکونیال و ریسه ای که به...
Gastric inhibitory polypeptide (GIP) exhibits potent insulinotropic effects on β-cells and anabolic effects on bone formation and fat accumulation. We explored the impact of reduced GIP levels in vivo on glucose homeostasis, bone formation, and fat accumulation in a novel GIP-GFP knock-in (KI) mouse. We generated GIP-GFP KI mice with a truncated prepro-GIP gene. The phenotype was assessed in he...
Recently, misuse or overuse of antibiotics has led to the antibiotic resistance problem, a global healthcare problem. Most virulence factors and biofilm formation in Pseudomonas aeruginosa are controlled by quorum sensing (QS). The inhibition QS system inhibitor molecules been suggested as novel alternative antivirulence approach which no need kill bacteria. In present study, inhibitory potenti...
Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies. Materials & Methods: After prep...
Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...
Defects in glucagon action can cause hyperplasia of islet α-cells, however, the underlying mechanisms remain largely to be elucidated. Mice homozygous for a glucagon-GFP knock-in allele (Gcg(gfp/gfp) ) completely lack proglucagon-derived peptides and exhibit hyperplasia of GFP-positive α-like cells. Expression of the transcription factor, aristaless-related homeobox (ARX), is also increased in ...
I want to thank the Royal Swedish Academy of Sciences and the Nobel Foundation for this amazing and surprising honor. At first I wondered why I, a biologist and a person with less than enviable college grades in chemistry, had been selected. Then I realized that this prize had actually been given to the GFP molecule, and I am one of its assistants. Thank you for letting me be part of the celebr...
The recombinant antibodies AF394, AF395, AF397, AK142, AK652, AN193 and AV441 detect the GFP protein by western blot.
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