A specific and sensitive multiplex PCR (mPCR) method was developed as a useful tool for the simultaneous detection of two important flatfish pathogens in marine aquaculture, Tenacibaculum maritimum and Edwardsiella tarda. In fish tissues, the average detection limit for these mPCR-amplified organisms was 2 × 10 ⁵ ± 0.2 CFU/g and 4 × 10 ⁵ ± 0.3 CFU/g, respectively. These values are similar or ev...

Gafrarium divaricatum (Gmelin) was exposed to sublethal concentrations of xylene (4.25 and 8.50 mg/l), benzene (4.35 and 8.70 mg/l) and gear oil-WSF (1 and 2%) for 30 days. There was a decline in condition index of the treated clams while moisture content showed an increase on day 30. Glycogen content of hepatopancreas of the clams treated with the pollutants registered an initial increase on d...

We studied the phylogeography of Eryngium alpinum by sequencing two intergenic chloroplast spacers, trnH-psbA and trnS-trnG (1322 bp). The sampling design included 36 populations and 397 individuals spanning the entire distribution range of the species, from France to Bosnia. Twenty-one haplotypes were characterized and polymorphism was observed both within and among populations. Population dif...

The invasive Argentine ant (Linepithema humile) is established worldwide and displaces native ant species. In northern California, however, the native winter ant (Prenolepis imparis) persists in invaded areas. We found that in aggressive interactions between the two species, P. imparis employs a potent defensive secretion. Field observations were conducted at P. imparis nest sites both in the p...

This multidisciplinary study is focused on the conservation and sustainable utilization of Lomelosia minoana (Dipsacaceae; subsp. asterusica) Eryngium ternatum (Apiaceae), three local endemic plants Crete (Greece) with economic interest. Using Geographical Information Systems open-source geodatabases, detailed ecological profiles were compiled to illustrate abiotic environmental conditions prev...