We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and PLAP purified from FRT cells is raft-associated in vitro when reconstit...

Numerous techniques have been presented by different researchers to analyze piled raft. In order to analyze pile foundation, soil can be modeled as spring, continuous medium, or porous media. Pile can also be modeled as spring or continuous medium. This study includes three main stages: a short description of different types of analysis methods of pile foundation, writing a computer program bas...

Large (>1600 microm), ingestively masticated particles of bermuda grass (Cynodon dactylon L. Pers.) leaf and stem labelled with 169Yb and 144Ce respectively were inserted into the rumen digesta raft of heifers grazing bermuda grass. The concentration of markers in digesta sampled from the raft and ventral rumen were monitored at regular intervals over approximately 144 h. The data from the two ...

Cell adhesion and spreading require integrins-mediated cell-extracellular matrix interaction. Integrins function through binding to extracellular matrix and subsequent clustering to initiate focal adhesion formation and actin cytoskeleton rearrangement. Lipid raft, a liquid ordered plasma membrane microdomain, has been reported to play major roles in membrane motility by regulating cell surface...

We propose a Byzantine Fault Tolerant variant of the Raft consensus algorithm, BFTRaft, inspired by the original Raft[1] algorithm and the Practical Byzantine Fault Tolerance algorithm[2]. BFT Raft maintains the safety, fault tolerance, and liveness properties of Raft in the presence of Byzantine faults, while also aiming towards to Raft’s goal of simplicity and understandability. We have imple...

Despite the importance of cholesterol in the formation and function of caveolar microdomains in plasma membranes, almost nothing is known regarding the structural properties, cholesterol dynamics or intracellular factors affecting caveolar cholesterol dynamics. A non-detergent method was employed to isolate caveolae/raft domains from purified plasma membranes of murine fibroblasts. A series of ...