background classification of cancer subtypes by means of microarray signatures is becoming increasingly difficult to ignore as a potential to transform pathological diagnosis nonetheless, measurement of indicator genes in routine practice appears to be arduous. in a preceding published study, we utilized real-time pcr measurement of indicator genes in acute lymphoid leukaemia (all) and acute my...

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

The human metapneumovirus (hMPV) is a new member of the Paramyxoviridae family associated with acute respiratory tract infections in humans. The objective of this study was to compare the sensitivity of real-time RT-PCR assays performed in a LightCycler instrument and designed to amplify the viral nucleoprotein (N), matrix (M), fusion (F), phosphoprotein (P), and polymerase (L) genes. In a firs...

2.Cusi, M.G., M. Valassina and P.E. Valensin. 1994. Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RTPCR of samples with low virus burden. BioTechniques 17:1034-1036. 3.Fiorenza, M.T. and F. Mangia. 1998. Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos. BioTechniques 24:618-623. 4.Huang, Z., M.J. Fasco and L.S. Kaminsky. 199...

F-specific RNA bacteriophages have been classified into four genogroups (GI, GII, GIII and GIV). It was suggested that two of these genogroups are more frequent in human excreta (GII and GIII) and the two other (GI and GIV) are specific for animal excreta. Real-time RT-PCR methods using TaqMan MGB probe were developed to detect the four genogroups. Primers and probes of each specific RT-PCR wer...