When a brine mixed with particles is frozen, some liquid water persists due to the freezing point depression caused by solute impurity, surface energy, and disjoining pressure (wetting forces). This unfrozen forms complex “liquid vein network” (LVN). However, details of process are still not fully understood, including permeability/tortuosity LVN, content at given temperature. Here, we have app...

ABSTRACT Plant parasitic nematodes cause severe agricultural damage in Northeast Brazil. Additionally, soil salinization, especially the semiarid region of Northeast, is another factor that limits crop yield. The study evaluated motility Meloidogyne enterolobii and Pratylenchus coffeae, vertical migration P. coffeae under saline conditions. Motility was assessed by submitting juveniles second s...

Gas-permeable membrane technology is useful to recover ammonia (NH3) from liquid manures. In this study, phosphorus (P) recovery via MgCl2 precipitation was enhanced by combining it with NH3 recovery through gas-permeable membranes. Anaerobically digested swine wastewater containing approximately 2300 mg NH4+-N L-1 and 450 mg P L-1 was treated using submerged membranes plus low-rate aeration to...

alpha and beta DNA polymerases (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) were isolated from nuclear and cytoplasmic fractions of rat livers exposed to a carcinogenic regimen with the hepatocarcinogen N-2-fluorenylacetamide and from 24-hr regenerating liver. The fidelity of polymerization of these enzymes was compared by determining the...

We have examined the effects of histone hyperacetylation upon nuclease digestion of nuclei and subsequent fractionation of chromosomal material in the presence of MgCl2. DNase I shows a maximum sensitivity towards hyperacetylated nuclei at somewhat elevated ionic strengths (150-200 mM NaCl), whereas micrococcal nuclease exhibits no specificity for acetylated nuclei over a broad range of ionic s...

Influenza A virus utilizes RNA throughout infection. Little is known, however, about the roles of RNA structures. A previous bioinformatics survey predicted multiple regions of influenza A virus that are likely to generate evolutionarily conserved and stable RNA structures. One predicted conserved structure is in the pre-mRNA coding for essential proteins, M1 and M2. This structure starts 79 nu...