هدف : اول پژوهش حاضر شناسایی مقوله‌ها و مفاهیم تجاری‌سازی دانش جهت ارائه یک الگوی مفهومی است. دوم آن آزمون تجربی تجاری سازی می‌باشد.مواد روش‌ها: با راهبرد ترکیبی از نوع اکتشافی انجام شد. مشارکت‌کنندگان در رؤسا، مدیران گروه استادان دانشگاه، متخصصان مراکز رشد دانشگاهی پارک علم فناوری استان گیلان بودند. برای پژوهش، مرحله کیفی30 نفر استفاده نمونه‌گیری هدفمند کمی، 230 روش تصادفی طبقه‌ای به‌عنوان نمو...

BACKGROUND The cAMP-dependent protein kinase A (PKA) plays a pivotal role in virtually all cells, there being a multitude of important target molecules that are substrates for PKA in cell signaling. The spatial-temporal dynamics of PKA activation in living cells has been made accessible by the development of clever biosensors that yield a FRET signal in response to the phosphorylation by PKA. A...

Acute insulin secretion determines the efficiency of glucose clearance. Moreover, impaired acute insulin release is characteristic of reduced glucose control in the prediabetic state. Incretin hormones, which increase β-cell cAMP, restore acute-phase insulin secretion and improve glucose control. To determine the physiological role of the cAMP-dependent protein kinase (PKA), a mouse model was d...

Coxiella burnetii is the bacterial agent of human Q fever, an acute, flu-like illness that can present as chronic endocarditis in immunocompromised individuals. Following aerosol-mediated transmission, C. burnetii replicates in alveolar macrophages in a unique phagolysosome-like parasitophorous vacuole (PV) required for survival. The mechanisms of C. burnetii intracellular survival are poorly d...

Protein kinase A (PKA) has been suggested to be spatially regulated in migrating cells due to its ability to control signaling events that are critical for polarized actin cytoskeletal dynamics. Here, using the fluorescence resonance energy transfer-based A-kinase activity reporter (AKAR1), we find that PKA activity gradients form with the strongest activity at the leading edge and are restrict...

Maf1 is an essential and specific mediator of transcriptional repression in the RNA polymerase (pol) III system. Maf1-dependent repression occurs in response to a wide range of conditions, suggesting that the protein itself is targeted by the major nutritional and stress-signaling pathways. We show that Maf1 is a substrate for cAMP-dependent PKA in vitro and is differentially phosphorylated on ...

We investigated the involvement of PKA and PKC signaling in a negatively reinforced operant learning paradigm in Aplysia, learning that food is inedible (LFI). In vivo injection of PKA or PKC inhibitors blocked long-term LFI memory formation. Moreover, a persistent phase of PKA activity, although not PKC activity, was necessary for long-term memory. Surprisingly, neither PKA nor PKC activity wa...

Bu çalışmada, Eskişehir ili Kırka ilçesinden temin edilen bor işletme tesisi proses katı atıklarının (PKA) sondaj çamurlarında kullanılabilirliği araştırılmıştır. Çalışma kapsamında Amerikan Petrol Enstitüsü (API) Spec. 13-A standardına göre spud tip çamur numuneleri hazırlanmıştır. Hazırlanan numunelerde farklı oranlarda (ağırlıkça %4-8) ve tane boyutlarında (75-50µm) PKA bentoniti katkılanmış...

The capsaicin receptor, VR1 (also known as TRPV1), is a ligand-gated ion channel expressed on nociceptive sensory neurons that responds to noxious thermal and chemical stimuli. Capsaicin responses in sensory neurons exhibit robust potentiation by cAMP-dependent protein kinase (PKA). In this study, we demonstrate that PKA reduces VR1 desensitization and directly phosphorylates VR1. In vitro phos...