Misfolded proteins in the endoplasmic reticulum (ER) are eliminated by a quality control system called ER-associated protein degradation (ERAD). However, it is unknown how misfolded proteins in the inner nuclear membrane (INM), a specialized ER subdomain, are degraded. We used a quantitative proteomics approach to reveal an ERAD branch required for INM protein quality control in yeast. This bra...

The nuclear envelope (NE) in animal cells comprises three structures: the nuclear membrane, the nuclear pore complex (NPC), and the lamina. The nuclear membrane is divided into the inner nuclear membrane (INM) and outer nuclear membrane (ONM) based on protein content, but the membranes are contiguous with each other and with the ER. The nuclear membrane covers the chromatin and restricts nuclea...

By exploiting the instantaneous normal mode (INM) analysis for models of flexible molecules, we investigate intermolecular and intramolecular vibrations of water from the atomic point of view. With two flexible SPC/E models, our investigations include three aspects about their INM spectra, which are separated into the unstable, intermolecular, bending, and stretching bands. First, the O- and H-...

The nuclear envelope is a barrier comprising outer and inner membranes that separate the cytoplasm from the nucleoplasm. The two membranes have different physical characteristics and protein compositions. The processes governing the stability of inner nuclear membrane (INM) proteins are not well characterized. In Saccharomyces cerevisiae, the INM Asi1-Asi3 complex, principally composed of integ...

Isoforms of importin-α have been identified in insect and human cells, and cross-linking experiments suggest that at least one isoform in each species participates in the targeting of integral membrane proteins to the inner nuclear membrane (INM). To directly test this hypothesis, an assay was developed using Saccharomyces cerevisiae. The data show that internal promoters are present within KAP...

هدف: با توجه به کاستی‌های موجود، نظام سیاست‌گذاری سازمانی فرایندی سیستماتیک، هدفمند و منظم برای تولید داده‌های حاصل از عملکرد، پردازش آن‌ها اطلاعات تبدیل آن دانش سیاستی نیاز دارد تا نسبت کم کیف تحقق اهداف سیاست‌ها، آثار پیامدهای موفقیت یا ناکامی‌شان آگاهی داشته تصمیم‌گیری بهره گیرد. ازاین‌رو مسئله اصلی که محقق تلاش رفع می‌پردازد تبیین این موضوع است سیاست‌ها راهبردهای کلان چه پیامدها آثاری در پی...