AIM To compare the costs and cost effectiveness of universal and targeted screening for the haemoglobinopathies; to compare the cost of two laboratory methods; and to estimate the cost effectiveness of programmes at different levels of prevalence and mix of haemoglobinopathy traits. METHODS A retrospective review of laboratory and follow up records to establish workload and costs, and estimat...

Electrophoretic methods can be used to identify meat of various animal species. The protein electrophoresis, especially the IEF of the sarcoplasmic proteins, is a wellestablished technique for species identification of raw fish and is used in the control of seafood authenticity. However, in the case of the analysis of heat-processed fish, the method is applicable only to those species which pos...

BACKGROUND α-1-Antitrypsin (A1AT) deficiency results from a genetic disorder at 2 common loci. Diagnosis requires quantification of A1AT and subsequent identification of the specific variant. The current algorithm of laboratory testing for the diagnosis of A1AT deficiency uses a combination of quantification (nephelometry), genotyping, and/or phenotyping. We developed a multiple reaction monito...

We investigate an axiomatization of the notion of common bel ief (knowledge) that makes use of no rules of inference (apart from Modus Ponens and Necessitat ion) and highl ight the property of the set of accessibi l i ty relat ions that characterizes each axiom. Mathernatics Subject Classif icat ion: 03845, 68T25.

The nonclassical class I HLA-E molecule folds in the presence of peptide ligands donated by the signal sequences of permissive class I HLA alleles, with the aid of TAP and tapasin. To identify HLA-E-specific Abs, four monoclonals of the previously described MEM series were screened by isoelectric focusing (IEF) blot and immunoprecipitation/IEF on >30 single-allele class I transfectants and HLA-...

points and for determining the presence of charged mojeties on unknown antibiotics using minimal(microgram)quantities ofcrude material. Such infbrmatlon would also be valuable for identifying the presence of known substances in an antibiotic screen. However, IEF has not been apPhed extensively to antibiotics because their smaller size makes detection by traditional staining techniques difficult.

Isoelectric Focusing in Immobilized pH Gradient Strips Using the IPGphor Unit: Sample In-Gel Rehydration Angelika Görg, Oliver Drews, and Walter Weiss This protocol was adapted from "IEF in IPG Strips Using the IPGphor Unit," contributed by Angelika Görg, Oliver Drews, and Walter Weiss, Chapter 16, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spr...

We review microfluidic devices designed for multidimensional sample analysis, with a primer on relevant theory, an emphasis on protein analysis, and an eye towards future improvements and challenges to the field. Image shows results of an on-chip IEF-CE separation of a protein mixture; unpublished surface plot data from A. E. Herr.

Methods Seventy fetuses at 19 to 28 weeks of gestation with an IEF and 63 controls were screened using two-dimensional and power Doppler echocardiography. DV wave velocities, DV velocity ratios, and diastolic time intervals were measured. The aortic peak velocity, pulmonary artery peak velocity, atrioventricular early-diastolic filling velocity (E), atrial contraction velocity (A), and E/A rati...

In a UK survey of the occurrence of extended spectrum beta-lactamases, 96 hospitals submitted a total of 3951 non-selected, non-duplicate isolates of Enterobacteriaceae from 100 patients in each hospital, 206 of these cultures being mixed and, therefore, discarded. These isolates were initially screened for strains likely to produce extended-spectrum beta-lactamases (ESBLs) by MIC determination...