نتایج جستجو برای: فراگمنتاسیون dna
تعداد نتایج: 507337 فیلتر نتایج به سال:
background: occupational and environmental exposures mostly represent complex mixture of genotoxic agents, however there is a wide variation in the specificity of biomarkers. exploration of correlations among biomarkers contributes to the further progress of molecular cancer epidemiology and to the selection of the optimal biomarkers for the investigation of human exposure to carcinogens. the a...
in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...
background: the present study was carried out to detect the infection of larval stages of trichobilharzia species in the snail lymnaea auricularia in northwestern iran based on dna analysis. methods: a total number of 320 snails of l. auricularia were sampled from four water-bodies located in the suburb of urmia city, north west iran, during may to november 2011. the snails were first microscop...
conclusions a significant direct correlation was observed between the viral dna copies present in patients’ cerumen and serum. however, the correlation between saliva viral load with serum and cerumen viral load was very low and inverse. these findings suggest that the presence of the hepatitis b virus (hbv) in non-invasive specimens (such as cerumen and saliva) should also be evaluated when mo...
هدف: هدف تولید یک سازه بیان کننده توالی یک ناحیه همپوشان از ژن ns3 از ویروس ایزوله ایرانی است. مواد و روشها: ابتدا بخش میانی ژن ns3 با روش nested-rt-pcr و با استفاده از سرم بیمار مبتلا به ژنوتیپ 1a ویروس هپاتیت c تکثیر شد. بعد از کلون آن درون ناقل کلونینگ ta و غربالگری کلونها براساس روش سفید- آبی و colony-pcr این کلونهای منتخب با روش تعیین توالی و هضم آنزیمی با bglii ارزیابی شد. توالی جدید ب...
samples from five sturgeon species including persian sturgeon (acipenser persicus), russian sturgeon (a. gueldenstaedti), great sturgeon (huso huso),ship sturgeon (a. nudi-ventris) and stellate sturgeon (a. stellatus) were collected from the south caspian sea. dna was extracted from the fins, eggs and skeleton muscle of the five species. rapd markers were used for identifying different kinds of...
dna methylation is an important biological process involving in human disease such as cancer insomia and diabetes. bisulfite sequencing (bs-seq) with next-generation technology is an accurate method for measuring dna methylation. bs-seq data analysis is a considerable way to recognize methylated cytosines and several tools have been developed to analysis bs-seq such as bs-seeker, b-solana, brat...
in order to perform breeding programs and improve production of native chickens, preserving genetic diversity in different areas of iran is important due to the reduced available population. genome sequencing is considered the most functional approach to determine the phylogeny relation between close populations. the aim of the present study was the evaluation of the phylogeny and genetic nucle...
استخراج dna بهصورت خالص، پایدار و با کمیت و کیفیت مناسب، گام مهمی در اجرای پژوهشهای بیولوژیکی میباشد. در این پژوهش، dna مربوط به نمونه خون 600 بلدرچین ژاپنی با روش نمکی تغییر یافته با پودر رختشویی و بدون استفاده از آنزیم پروتئیناز k استخراج شد. نمونههای dna بهدست آمده در مراحل آمادهسازی کتابخانه ژنومی، با نانودراپ، فلئورومتر، بیوآنالیزر، الکتروفورز، pcr، هضم با آنزیمهای آندونوکلئاز، اتصا...
in this study, aggregation behavior of a water soluble cationic metalloporphyrin, meso-tetrakis (2,3,5,6-tetrafluoro-n,n´,n´´-trimethyl ammonium phenyl) porphyrin nickel(ii) [ni(ii)(tf4tmapp)]4+ is investigated in 5 mm aqueous phosphate buffer of ph 7.0 at 25.0 °c and various ionic strengths using optical absorption and resonance light scattering spectroscopic methods. the results suggest that ...
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