نتایج جستجو برای: فراگمنتاسیون dna

تعداد نتایج: 507337  

Journal: :iranian journal of cancer prevention 0
sudha sellappa dept. of biotechnology, school of life sciences, karpagam university, coimbatore, tamilnadu, india gem gemitha dept. of biotechnology, school of life sciences, karpagam university, coimbatore, tamilnadu, india

background: occupational and environmental exposures mostly represent complex mixture of genotoxic agents, however there is a wide variation in the specificity of biomarkers. exploration of correlations among biomarkers contributes to the further progress of molecular cancer epidemiology and to the selection of the optimal biomarkers for the investigation of human exposure to carcinogens. the a...

Journal: :applied biotechnology reports 0
mohammad ebrahim minaei department of biology, imam hossein university, tehran, iran mojtaba saadati department of biology, imam hossein university, tehran, iran mostafa najafi department of chemistry, imam hossein university, tehran, iran hossein honari department of biology, imam hossein university, tehran, iran

in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...

Journal: :iranian journal of parasitology 0
mohammad yakhchali asaad hosseinpanahi reza malekzadeh-viayeh

background: the present study was carried out to detect the infection of larval stages of trichobilharzia species in the snail lymnaea auricularia in northwestern iran based on dna analysis. methods: a total number of 320 snails of l. auricularia were sampled from four water-bodies located in the suburb of urmia city, north west iran, during may to november 2011. the snails were first microscop...

Journal: :hepatitis monthly 0
elaheh gholami parizad clinical microbiology research centre, ilam university of medical sciences, ilam, ir iran eskandar gholami parizad public health department, psychosocial injuries prevention research centre, ilam university of medical sciences, ilam, ir iran; public health department, psychosocial injuries prevention research centre, ilam university of medical sciences, ilam, ir iran. tel: +98-8432240404, fax: +98-8432240404 afra khosravi clinical microbiology research centre, ilam university of medical sciences, ilam, ir iran mansour amraei department of clinical physiology, research centre, ilam university of medical sciences, ilam, ir iran azar valizadeh clinical microbiology research centre, ilam university of medical sciences, ilam, ir iran abdoullah davoudian department of clinical immunology, ilam university of medical sciences, ilam, ir iran

conclusions a significant direct correlation was observed between the viral dna copies present in patients’ cerumen and serum. however, the correlation between saliva viral load with serum and cerumen viral load was very low and inverse. these findings suggest that the presence of the hepatitis b virus (hbv) in non-invasive specimens (such as cerumen and saliva) should also be evaluated when mo...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
seyed-younes hosseini farzaneh sabahi department of virology, faculty of medical sciences, tarbiat modares university, tehran mohamad hossein modarresi seyed mohammad moazzeni department of immunology, school of medical sciences, tarbiat modares university, tehran, iran mehdi saberi firoozi mehrdad ravanshad department of virology, school of medical sciences, tarbiat modares university, tehran, iran davood mehrabani

هدف: هدف تولید یک سازه بیان کننده توالی یک ناحیه همپوشان از ژن ns3 از ویروس ایزوله ایرانی است. مواد و روش‏ها: ابتدا بخش میانی ژن ns3 با روش nested-rt-pcr و با استفاده از سرم بیمار مبتلا به ژنوتیپ 1a ویروس هپاتیت c تکثیر شد. بعد از کلون آن درون ناقل کلونینگ ta و غربالگری کلون‏ها براساس روش سفید- آبی و colony-pcr این کلون‏های منتخب با روش تعیین توالی و هضم آنزیمی با bglii ارزیابی شد. توالی جدید ب...

Journal: :journal of agricultural science and technology 2010
s. rezvani gilkolaie

samples from five sturgeon species including persian sturgeon (acipenser persicus), russian sturgeon (a. gueldenstaedti), great sturgeon (huso huso),ship sturgeon (a. nudi-ventris) and stellate sturgeon (a. stellatus) were collected from the south caspian sea. dna was extracted from the fins, eggs and skeleton muscle of the five species. rapd markers were used for identifying different kinds of...

Journal: :journal of advances in computer research 2014
nadia barjaste reza nadimi majid alipour

dna methylation is an important biological process involving in human disease such as cancer insomia and diabetes. bisulfite sequencing (bs-seq) with next-generation technology is an accurate method for measuring dna methylation. bs-seq data analysis is a considerable way to recognize methylated cytosines and several tools have been developed to analysis bs-seq such as bs-seeker, b-solana, brat...

Journal: :iranian journal of applied animal science 2015
a. afsharian m.r. nassiri

in order to perform breeding programs and improve production of native chickens, preserving genetic diversity in different areas of iran is important due to the reduced available population. genome sequencing is considered the most functional approach to determine the phylogeny relation between close populations. the aim of the present study was the evaluation of the phylogeny and genetic nucle...

ژورنال: :دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی) 2012
احمد احمدی حسن مهربانی یگانه سید رضا میرایی آشتیانی اردشیر نجاتی جوارمی عباس پاکدل

استخراج dna به­صورت خالص، پایدار و با کمیت و کیفیت مناسب، گام مهمی در اجرای پژوهش­های بیولوژیکی می­باشد. در این پژوهش، dna مربوط به نمونه خون 600 بلدرچین ژاپنی با روش نمکی تغییر یافته با پودر رختشویی و بدون استفاده از آنزیم پروتئیناز k استخراج شد. نمونه­های dna به­دست آمده در مراحل آماده­سازی کتابخانه ژنومی، با نانودراپ، فلئورومتر، بیوآنالیزر، الکتروفورز، pcr، هضم با آنزیم­های آندونوکلئاز، اتصا...

Journal: :physical chemistry research 2015
nahid rasouli nasrin sohrabi* fatemea fateminasab

in this study, aggregation behavior of a water soluble cationic metalloporphyrin, meso-tetrakis (2,3,5,6-tetrafluoro-n,n´,n´´-trimethyl ammonium phenyl) porphyrin nickel(ii) [ni(ii)(tf4tmapp)]4+ is investigated in 5 mm aqueous phosphate buffer of ph 7.0 at 25.0 °c and various ionic strengths using optical absorption and resonance light scattering spectroscopic methods. the results suggest that ...

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