Laminin, a major component of basement membranes, is a large glycoprotein consisting of three disulfide-bonded subunits, A, B1, and B2. We have isolated and sequenced a Drosophila laminin B2 chain cDNA clone that spans 5737 nucleotides. The deduced amino acid sequence predicts that the mature and nonglycosylated polypeptide has a chain length of 1606 residues (Mr = 178,665). This B2 chain conta...

This paper gives an overview of our tutoring system, B2. It describes the natural language and knowledge representation components of B2, and our approach to the representation of questions and requests. The domain that we have developed most thoroughly helps medical students learn a statistical model for medical diagnosis. Many of the examples will be taken from this domain. According to B2’s ...

We have measured the positions of H2O masers in Sgr B2, a massive star forming region in the Galactic center, relative to an extragalactic radio source with the Very Long Baseline Array. The positions measured at 12 epochs over a time span of one year yield the trigonometric parallax of Sgr B2 and hence a distance to the Galactic center, R0, of R0 = 7.9 +0.8 −0.7 kpc. The proper motion of Sgr B...

Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P. philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P. philadelphia only contains ...

Human peripheral blood polymorphonuclear leukocytes were stimulated to generate thromboxane B2 in a time- and concentration-dependent fashion upon exposure to serum-treated zymosan particles. Conversion by stimulated PMN of [14C] arachidonic acid to [14C]thromboxane B2 was confirmed by thin-layer radiochromatography, radio-gas chromatography, and mass spectrometry. Generation of thromboxane B2 ...

Given a combinatorial designD with block set B, its traditional block-intersection graph GD is the graph having vertex set B such that two vertices b1 and b2 are adjacent if and only if b1 and b2 have non-empty intersection. In this paper we consider the S-block-intersection graph, in which two vertices b1 and b2 are adjacent if and only if |b1 ∩ b2| ∈ S. As our main result we prove that {1, 2,...

Transcription of the transforming growth factor-b2 (TGF-b2) gene is dependent on a cAMP-response element/activating transcription factor (CRE/ATF) site that is bound by CREB and ATF-1 as well as an E-box motif that is bound by upstream stimulatory factors 1 and 2 (USF1 and USF2). To identify additional factors involved in the expression of the TGF-b2 gene, we employed F9 embryonal carcinoma (EC...

The GABA(B) (gamma-aminobutyric acid-B) receptor is composed of two subunits, GABA(B1) and GABA(B2). Both subunits share structural homology with other class-III G-protein-coupled receptors. They contain two main domains, a heptahelical domain typical of all G-protein-coupled receptors and a large ECD (extracellular domain). It has not been demonstrated whether the association of these two subu...

In chicken, three structurally distinct nuclear lamin proteins have been described. According to their migration on two-dimensional gels, these proteins have been designated as lamins A, B1, and B2. To investigate the functional relationship between chicken lamins and their mammalian counterparts, we have examined here the state of individual chicken lamin proteins during mitosis. Current model...

This paper describes B2, an extension to BANTER. BANTER is a tutoring shell that allows medical students to practice their decision-making skills by considering a number of medical cases that diier from each other in a controlled manner. The underlying inference engine uses a Bayesian network. B2 provides a natural language interface to BANTER that is consistent with principles for cooperative ...