We consider fixed recombinant inbred lines (RILs) derived either by selfing or by full-sib mating; when applicable, we also consider intermated recombinant inbreds (IRIs). First, we show that the usual estimate of recombination fraction based on RIL data is biased, and we provide an estimate where the major part of that bias is removed. Second, we derive simple formulas to compute the frequenci...

Journal of Plant Registrations, Vol 2, No. 2, May 2008 137 Three F8–derived breeding lines, W6 26740 (RIL 846_34; GP-96, PI 652444), W6 26743 (RIL 846_40; GP-97, PI652445), and W6 26745 (RIL 847_36; GP-98, PI 652446), of green pea (Pisum sativum L.) were selected from a recombinant inbred line population developed by the USDA–ARS in 2000. These lines are unique in combining high levels of resis...

The author observed a fact that the incubated splenocytes of the Fischer strain rats with recombinant human interleukin-2 (rIL-2) developed lytic activity to syngeneic natural killer-resistant 9L-glioma cells by a 4-h Cr release assay. The induction of their lymphokine-activated killer (LAK) cell was as follows. 1) When 5 •~ 106/ml splenocytes were cultured with various concentrations of rIL-2 ...

We studied the effects of natural and recombinant human IL-2 (rIL-2) on secretion of prostacyclin (PGI2), vWf, and tissue-type plasminogen activator (tPA). IL-2 elicited a steady increase in PGI2 synthesis by cultured human umbilical vein endothelial cells (HUVECS) and bovine aortic endothelial cells but had no effect on vWf or tPA. Both purified natural IL-2 (nIL-2) and rIL-2 induced significa...

The diversity of infectious agents capable of inducing meningitis and blood-brain barrier (BBB) injury suggests the potential for a common host mediator. The inflammatory polypeptides, IL-1 and TNF, were tested in an experimental rat model as candidate mediators for induction of meningitis and BBB injury. Intracisternal challenge of rIL-1 beta into rats induced neutrophil emigration into cerebr...