Bacillus thuringiensis (Bt) is widely used to control agricultural and forestry pests, though there are only a few available complete genome sequences of the Bt reference strain. Here, we report the complete genome sequence of B. thuringiensis serovar rongseni reference strain SCG04-02, which is toxic to Plutella xylostella.

Bacillus thuringiensis has been widely used as a biopesticide for a long time. Here we report the finished and annotated genome sequence of B. thuringiensis mutant strain BMB171, an acrystalliferous mutant strain with a high transformation frequency obtained and stocked in our laboratory.

Bacillus thuringiensis is widely used in producing ecofriendly microbial agents for the purpose of controlling insect pests. In this study, we determined the complete genome sequence of B. thuringiensis subsp. jinghongiensis reference strain YGd22-03, which contains three cry genes and one cerecidin biosynthetic gene cluster.

A method is described for the purification of Bacillus thuringiensis protein crystals by Ludox gradient centrifugation. This method is simple, inexpensive, fast, and efficient compared with other techniques. It has been successfully used to purify and characterize the protein crystals from several B. thuringiensis strains.

The effects of Bacillus thuringiensis subsp. tenebrionis applications and early harvest of potatoes on reducing the size of overwintering Colorado potato beetle, Leptinotarsa decemlineata (Say), populations in eastern North Carolina were investigated over 3 yr. In large-plot (0.04 ha) experiments, potato beetle densities and defoliation levels in plots that were either treated with B. thuringie...

During speculation, Bacillus thuringiensis produces parasporal inclusions with insecticidal activity. The parasporal inclusions produced by most subspecies of B. thuringiensis are active only against the larvae of a few lepidopteran insects. Lepidopteran-active parasporal inclusions are usually bipyramidal crystals composed of one or more 130x lCP-l^Ox 10 Mr polypeptides. These polypeptides are...

Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resistance to B. thuringiensis in open-field populations. The only known mechanism o...

Bacillus thuringiensis serovar israelensis is well known for its mosquitocidal activity and has long been used as a biopesticide. Herein, we present the genome sequence of B. thuringiensis serovar israelensis strain 4Q7, a plasmid-cured derivative with higher transformation efficiency than wild types.

Bacillus thuringiensis crystal proteins of the Cry34 and Cry35 classes function as binary toxins showing activity on the western corn rootworm, Diabrotica virgifera virgifera LeConte. We surveyed 6,499 B. thuringiensis isolates by hybridization for sequences related to cry35A genes, identifying 78 strains. Proteins of the appropriate molecular mass (ca. 44 kDa) for Cry35 were observed in 42 of ...