Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

Codon bias refers to the differences in the frequency of occurrence of synonymous codons in coding DNA. Pattern of codon and optimum codon utilization is significantly different between the lives. This difference is due to the long term function of natural selection and evolution process. Genetics drift, mutation and regulation of gene expression are the main reasons for codon bias. In this stu...

Pyruvate orthophosphate dikinase, phosphoenolpyruvate carboxylase, and NADP-malate dehydrogenase function in a series of reactions for fixing Con in mesophyll cells and NADP-malic enzyme (ME) catalyzes the production of CO, and NADPH in bundle sheath cells of maize which is a NADP-ME type C4 plant. Northern blot analyses with cDNA clones for pyruvate orthophosphate dikinase and phosphoenolpyr...

A broad spectrum of health benefits from intermittent fasting have been reported in studies on animal models and human subjects. However, the underlying mechanisms these beneficial effects remain largely elusive. The present study aimed to explore potential mode action mouse with a focus liver. C57BL/6 mice were subjected or ad libitum feeding as controls. It was determined that 12 h d...