The real-time polymerase chain reaction (real-time PCR) is a recent modification to PCR (UNIT 10.2) that is rapidly changing the nature of how biomedical science research is conducted. It was first introduced in 1992 by Higuchi and coworkers and has seen a rapid increase in its use since (Higuchi et al., 1992, 1993). Real-time PCR allows precise quantification of specific nucleic acids in a com...

Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...

trichosanthes kirilowii, an important economic plant in china, is sensitive to chilling. to investigate the effect of abscisic acid (aba) treatment on the chilling tolerance of trichosanthes kirilowii maxim, different concentrations of aba were sprayed on trichosanthes kirilowii leaves and the resulting catalase (cat) and superoxide dismutase (sod) activities and the malondialdehyde (mda) conte...

objective: prostate cancer is one of the most common cancer in the developed countries. most of cancer deaths are due to development of metastasis. hence, prevention of metastasis is critical. silibinin is a flavonoid component that inhibits cell proliferation and causes cell death of human prostate cancer. in this study, the expression of cd82 gene in pc-3 cells treated with escalating concent...

background: cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. it is caused by certain strains of the species vibrio cholerae which can also cause mild or in apparent infections. the aim of this study is the evaluation of capsaicin, as a potential in...