icterus developed, and the patient died of shock and multiorgan failure 9 hours after hospitalization, despite treatment with a mixture of antibiotics, which contained doxycycline. Rickettsiae of the spotted fever group were isolated by the shell vial technique from the blood of the three patients. Sequences of polymerase chain reaction-amplified fragments of 16SrRNA (1440 bp), citrate synthase...

Two bacterial strains designated C9 and C11 were isolated and characterized from black tiger shrimp Penaeus monodon maintained in laboratory conditions. The shrimp exhibited tiny white spots along with clinical signs of bacterial infection, probably, due to suboptimal water quality and resulting stress. Absence of white spot syndrome virus was confirmed by PCR. Bacterial isolates obtained from ...

Human stomach is the only known natural habitat of Helicobacter pylori (Hp), a major bacterial pathogen that causes different gastroduodenal diseases. Despite this, the impact of Hp on the diversity and the composition of the gastric microbiota has been poorly studied. In this study, we have analyzed the culturable gastric microbiota of 215 Malaysian patients, including 131 Hp positive and 84 H...

Rhizosphere chemistry is the sum of root exudation chemicals, their breakdown products and the microbial products of soil-derived chemicals. To date, most studies about root exudation chemistry are based on sterile cultivation systems, which limits the discovery of microbial breakdown products that act as semiochemicals and shape microbial rhizosphere communities. Here, we present a method for ...

OBJECTIVE To develop a rapid and definite diagnostic test of bacterial enteritis caused by pathogenic enterobacteria, the most frequent etiologic agent of infectious enteritis in the world. METHODS A set of conventional PCR assays were applied to detect and identify salmonella, shigella, and E. coli O157:H7 directly from pure culture and fecal samples. The general primers of pathogenic entero...

Leprosy is a chronic dermato-neurological disease caused by infection with Mycobacterium leprae. In 2013 almost 200,000 new cases of leprosy were detected around the world. Since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. Although leprosy is one of the oldest records of human disease, the mechanisms involved with ...

The bacterial and archaeal communities of two full-scale biogas producing plants (P1 P2), associated with a 999 kW cogeneration unit, both located in North Italy, were analyzed at start up fully operating phases, by means various molecular approaches: (i) Automated Ribosomal Intergenic Spacer Analysis; (ii) cloning sequencing PCR amplicons genes 16Srrna mcrA; (iii) 16S rDNA high throughput next...

Vitiligo is mediated by T cells targeting melanocyte antigens. Depigmentation in vitiligo-prone mice can be accelerated or delayed oral antibiotics vitiligo-prone, but not healthy mice. We questioned whether lifestyle factors influence microbiome composition and the development of human vitiligo. Patients controls matched for age, sex skin type were consented before administering dietary questi...

Plant growth promoting rhizobacteria (PGPR) have been extensively employed as biofertilizers to enhance the soil nutrition for several crop plants. Rhizobacteria with beneficial effects plants could therefore be used reduce dependence on synthetic chemical fertilizers in conventional agriculture. Within this study, we explored isolation of potential PGPR groundnut from agricultural fields Saura...

Background: Polycyclic aromatic hydrocarbons (PAHs) have detrimental effects on human, ecosystem, and biodiversity. Bioremediation is an option that has been used to remediate reduce the risk of contaminants such as PAHs. Microorganisms are readily available screen can be rapidly identified in many extreme environmental conditions. Mycobacteria a great potential for production bioactive compoun...