Localized hydroxyl radical probing has been used to explore the rRNA neighborhood around a unique position in the structure of the Escherichia coli 30S ribosomal subunit. Fe(II) was attached to ribosomal protein S4 at Cys-31 via the reagent 1-(p-bromoacetamidobenzyl)-EDTA. [FeCys31]S4 was then complexed with 16S rRNA or incorporated into active 30S ribosomal subunits by in vitro reconstitution ...

A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and subsequent affinity chromatography. It was tested with ribosomes mutated at the 23 S rRNA position 2655 (the elongation factor (EF)-G binding site). R...

Lime is used to reduce soil acidification in agricultural soils. However, its effects on the microbial community are not well understood. Additionally, known be influenced by fertilizers. question remains whether liming influences magnitude of fertilizers' impact communities. Therefore, an incubation experiment was performed understand effect lime application (pH = 6.5 and 5.5 for soils with wi...

Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as starter culture Chinese Baijiu production. Bacillus Acinetobacter , two dominant microbial genera in Daqu, produce enzymes organic acids that influence the quality. However, there are no rapid analytical methods detecting . We designed primers specific to perform genetic comparisons using 16 S rRNA. ...

A sequence-specific modification of the human 5.8 S rRNA in isolated 60 S subunits, non-programmed 80 S ribosomes and ribosomes complexed with mRNA and tRNAs was studied with the use of a derivative of the nonaribonucleotide UCUGUGUUU bearing a perfluorophenylazide group on the C-5 atom of the 5'-terminal uridine. Part of the oligonucleotide moiety of the derivative was complementary to the 5.8...

Carbofuran is frequently applied to crop fields combat insects globally causing serious environmental pollution. In this study, the enhanced degradation of carbofuran in soil by augmentation with Bacillus sp. DT1 was determined. The results showed that bacterial strain increased almost 30% compared attenuation after 30 days at an initial concentration 10 mg/kg dry soil. half-life values insecti...

Ribosome biogenesis in Saccharomyces cerevisiae occurs primarily in a specialized nuclear compartment termed the nucleolus within which the rRNA genes are transcribed by RNA polymerase I into a large 35 S rRNA precursor. The ensuing association/dissociation and catalytic activity of numerous trans-acting protein factors, RNAs and ribosomal proteins ultimately leads to the maturation of the prec...