Type II methanotrophs produce polyhydroxybutyrate (PHB), while Type I methanotrophs do not. A laboratory-scale fluidized bed reactor was initially inoculated with a Type II Methylocystis-like dominated culture. At elevated levels of dissolved oxygen (DO, 9 mg/L), pH of 6.2-6.5 with nitrate as the N-source, a Methylobacter-like Type I methanotroph became dominant within the biofilms which did no...

Poly-3-hydroxy butyrate (PHB) is a copolymer of polyhydroxyalkonate family. It accumulates in the intracellular granules of bacteria under nitrogen limiting environment. The PHB producing bacteria was isolated from farm soil by serial dilution method. The Sudan Black B staining and Nile Blue A staining were carried out to confirm that the isolated strain was capable of producing PHB. Further, i...

A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear...

Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)-based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence,...

BACKGROUND Corynebacterium crenatum SYPA 5 is the industrial strain for L-arginine production. Poly-β-hydroxybutyrate (PHB) is a kind of biopolymer stored as bacterial reserve materials for carbon and energy. The introduction of the PHB synthesis pathway into several strains can regulate the global metabolic pathway. In addition, both the pathways of PHB and L-arginine biosynthesis in the cells...

The intended circular economy for plastics envisages that they will be partially replaced by bio-based polymers in the future. In this work, natural polyester polyhydroxybutyrate (PHB) was produced Azohydromonas lata using cheese whey (CW) as a low-cost substrate. Initially, CW evaluated sole carbon source PHB production; it found to efficient and comparable production with pure sugars, such sa...

In this study, blends of polypropylene (PP) with polylactic acid (PLA) and polyhydroxybutyrate (PHB) biopolymers wood flour were prepared, Fuzzy Grey Multi-Criteria Decision-Making (MCDM) methods used to determine the best properties. The physical, mechanical, thermal, structural, morphological properties composites determined. obtained results showed that PLA generally improved mechanical PP c...