نتایج جستجو برای: انگشت نگاری dgge

تعداد نتایج: 7753  

Journal: :FEMS microbiology ecology 2009
Rui Zhang Stanley C K Lau Jang-Seu Ki Vengatesen Thiyagarajan Pei-Yuan Qian

Bacterioplankton community structures under contrasting subtropical marine environments (Hong Kong waters) were analyzed using 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of predominant bands for samples collected bimonthly from 2004 to 2006 at five stations. Generally, bacterial abundance was significantly higher in the summer than in the winter. The ...

Journal: :The Journal of nutrition 2013
Catarina D Simões Johanna Maukonen Jaakko Kaprio Aila Rissanen Kirsi H Pietiläinen Maria Saarela

The impact of diet on the gut microbiota has usually been assessed by subjecting people to the same controlled diet and thereafter following the shifts in the microbiota. In the present study, we used habitual dietary intake, clinical data, quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) to characterize the stool microbiota of Finnish monozygotic twins...

2014
M. D. Carro

The aim of this study was to compare automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques to assess bacterial diversity in the rumen of sheep. Sheep were fed 2 diets with 70% of either alfalfa hay or grass hay, and the solid (SOL) and liquid (LIQ) phases of the rumen were sampled immediately before feeding (0 h) and at 4 and 8 h po...

2011
Nelli J. Beecroft Feng Zhao John R. Varcoe Alfred E. Thumser Claudio Avignone-Rossa

38 The performance and dynamics of the bacterial communities in the biofilm and suspended 39 culture in the anode chamber of sucrose-fed microbial fuel cells (MFCs) were studied by using 40 Denaturing Gradient Gel Electrophoresis (DGGE) of PCR-amplified partial 16S rRNA genes 41 followed by species identification by sequencing. The power density of MFCs was correlated to 42 the relative proport...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1992
J R Newmark D O Hardy D C Tonb B S Carter J I Epstein W B Isaacs T R Brown E R Barrack

We screened human prostate cancer tissues for the presence of somatic mutations in the hormone binding domain of the androgen receptor (AR) gene. Exons E-H were amplified from genomic DNA using the polymerase chain reaction and analyzed by denaturing gradient gel electrophoresis (DGGE), which separates DNA fragments that differ by only a single base. We detected a mutation in exon E of the horm...

Journal: :Journal of environmental sciences 2010
Likun Gu Zhihui Bai Bo Jin Qing Hu Huili Wang Guoqiang Zhuang Hongxun Zhang

Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone li...

ژورنال: :زیست فناوری گیاهان زراعی 0
میثم علی زاده دانش آموخته کارشناسی ارشد بیوتکنولوژی کشاورزی دانشگاه پیام نور، تهران قربانعلی نعمت زاده استاد پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری محمد علی ابراهیمی استادیار گروه بیوتکنولوژی کشاورزی دانشگاه پیام نور، تهران، ایران سیدحمید رضا هاشمی دانشجوی دکترای پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

برنج غذای عمده و منبع اصلی کربوهیدرات برای بیش از یک سوم از جمعیت جهان است. این گیاه همچنین جزء غذاهای اصلی مردم ایران محسوب می­شود. در این پژوهش به منظور ارزیابی و انگشت­نگاری تعدادی ژرم­پلاسم برنج کشور که شامل 28 رقم محلی و 19 رقم خارجی بوده است، از نشانگرهای مولکولی aflp استفاده گردید. با استفاده از 10 ترکیب آغازگر، تعداد 675 نوار (باند) به دست آمد که تعداد 429 نوار ­(5/63%) چندشکلی نشان داد...

2009
Tim Lachnit Martina Blümel Johannes F. Imhoff Martin Wahl

Epibacterial communities on thalli of the algal species Fucus serratus, Fucus vesiculosus, Laminaria saccharina, Ulva compressa, Delesseria sanguinea and Phycodrys rubens were analysed using 16S ribosomal RNA gene-based DGGE. Individuals of all species were collected in the Kiel Fjord (Baltic Sea) and in the rocky intertidal of Helgoland (North Sea). DGGE gels as well as cluster and multidimens...

Journal: :Geobiology 2013
A N Roychoudhury D Cowan D Porter A Valverde

Here, we report on the spatial and temporal variation in sulphate-reducing bacterial community structure and activity in three hypersaline coastal pans. Community structure was determined using denaturing gradient gel electrophoresis (DGGE). Cluster analysis of DGGE patterns indicated that similar microbial populations were generally found in individual pans but varied from one pan to the other...

2015
Siele Ceuppens Stefanie Delbeke Dieter De Coninck Jolien Boussemaere Nico Boon Mieke Uyttendaele Paul B. Tchounwou

Fresh herbs such as basil constitute an important food commodity worldwide. Basil provides considerable culinary and health benefits, but has also been implicated in foodborne illnesses. The naturally occurring bacterial community on basil leaves is currently unknown, so the epiphytic bacterial community was investigated using the culture-independent techniques denaturing gradient gel electroph...

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