One week of solitary confinement of prison inmates produced significant changes in their EEG frequency and visual evoked potentials (VEP) that parallel those reported in laboratory studies of sensory deprivation. EEG frequency declined in a nonlinear manner over the period. VEP latency, which decreased with continued solitary confinement, was shorter for these 5s than for control 5s whose VEP l...

esophageal squamous cell carcinoma (escc) is a deadly cancer with poor prognosis. in this regard, early diagnosis is of vital importance to cure the tumor in its early stages. novel cancer diagnostic and therapeutic approaches have been recently introduced based on micrornas (mirnas). also, accurate normalization using appropriate reference genes is a critical step in mirna expression studies. ...

The 5S method is a sophisticated Japanese tool for optimisation and improvement of the production process. The purpose of applying the 5S method is to increase the efficiency on the micro-level, keeping workplace clean, in order and accessible. The results are reflected in drastic increase of safety, space utilization, productivity, pride and Kaizen thinking; and drastic reduction of waste, def...

Most of the ribosomal RNA genes of the yeast Saccharomyces cerevisiae are about 9 kilobases (kb) in size and encode both the 35S rRNA (processed to produce the 25S, 18S, and 5.8S species) and 5S rRNA. These genes are arranged in a single tandem array of 100 repeats. Below, we present evidence that at the centromere-distal end of this array is a tandem arrangement of a different type of rRNA gen...

The synthesis of (4R,5R)-streptopyrrolidine (1), (4S,5R)-streptopyrrolidine (2) (4R,5S)-streptopyrrolidine (3) and (4S,5S)-streptopyrrolidine (4) have been achieved in a concise and highly efficient manner via a highly stereoselective aldol type reaction with the trimethylsilyl enolate of ethyl acetate and Lewis acid mediated lactamization as the key reactions in ≈42% yield over six steps start...

5S rRNA is uniquely positioned so as to link together all of the functional centers of the ribosome. Previous studies have supported the hypothesis that 5S rRNA acts as a physical transducer of information, facilitating communication between the different functional centers and coordinating of the multiple events catalyzed by the ribosome. Here, we present a synthesis of both structural and gen...

Three small RNAs of the cytoplasmic 8OS ribosomes of the green unicellular alga Chlamydomonas reinhardii have been sequenced. They include two species of ribosomal 5S RNA, a major and a minor one of 122 and 121 nucleotides respectively, which differ from each other by 17 bases, and also the ribosomal 5.8S RNA of 156 nucleotides. Novel structural features can be recognized in the 5S RNAs of C. r...

Zinc is essential for growth and reproduction of animals and i t has been suggested that the most critical requirement for Zn is for alteration in the genetic expression of cells (Chesters, 1978). However, in EDTA-treated lymphocyte cultures, lack of Zn delayed maturation of 32s ribosomal RNA precursor to 28s ribosomal RNA and reduced survival of the 28s RNA (Chesters, 1975). This alteration in...

We previously reported the chemical synthesis of oligonucleotides containing thymine glycol, a major form of oxidative DNA damage. In the preparation of the phosphoramidite building block, the predominant product of the osmium tetroxide oxidation of protected thymidine was (5R,6S)-thymidine glycol. To obtain the building block of the other isomer, (5S,6R)-thymidine glycol, in an amount sufficie...

Compounds that interact with microtubules, such as paclitaxel, have been shown to possess protective properties against beta-amyloid (Abeta) induced neurodegeneration associated with Alzheimer's disease. In this work, the novel agent (3R,5S,7as)-(3,5-bis(4-fluorophenyl)tetrahydro-1H-oxazolo[3,4-c]oxazol-7a-yl)methanol was investigated for effectiveness in protecting neurons against several toxi...