The amplification efficiencies of several polymerase chain reaction (PCR) enzymes were compared using real-time quantitative PCR with SYBR Green I detection. Amplification data collected during the exponential phase of PCR are highly reproducible, and PCR enzyme performance comparisons based upon efficiency measurements are considerably more accurate than those based on endpoint analysis. DNA p...

UNLABELLED PREMISE OF THE STUDY Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material. A DNA polymerase that is tolerant to inhibitors improves PCR results. • METHODS AND RESULTS A novel DNA amplification system, including a DNA polymerase engineered via directed evolution for improved tolera...

The introduction of polymerase chain reaction (PCR) into the forensic field has greatly extended the ability to analyze DNA from small or degraded samples. However, one significant problem with PCR analysis is the sensitivity of Taq Polymerase to inhibitors found in many substrates commonly encountered with evidentiary materials. We hypothesize that the most problematic of these compounds inter...

The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. We have examined the oligomerization of a MutS protein from Thermus aquaticus that binds to heteroduplex DNAs at elevated temperatures. Analytical gel filtration, cross-linking of MutS protein with disuccinimidyl suberate, light scattering, and matrix-assisted laser desorption/ionization time-of-f...

هدف: هدف از این مطالعه بررسی اثر سینامیک‌اسید (CA) بر برخی خصوصیات فیزیولوژیکی و میزان تحمل‌به‌شوری گیاه‌تنباکو (Nicotiana rustica L.) می‌باشد.مواد روش‌ها: در قطعات جداکشت گیاه تنباکو رشد‌یافته محیط MS به محیط‌های کشت حاوی (غلظت‌های 0، 5، 10 20 میلی‌گرم‌در‌لیتر) نمک NaCl 100 200 میلی‌مولار) منتقل‌گردید. پس چهار هفته فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز (PAL) تیروزین‌آمونیالیاز (TAL) ، ترکیبات‌فن...

In an order-driven financial market, the price of a asset is discovered through interaction orders - requests to buy or sell at particular that are posted public limit order book (LOB). Therefore, LOB data extremely valuable for modelling market dynamics. However, not freely accessible, which poses challenge participants and researchers wishing exploit this information. Fortunately, trades quot...

DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli. Purification requires 8 to 10 h and entails heat treating and clearing the E. coli lysate, followed by precipitatio...

Amplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChip) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain de...

Objective. It has been stated that brain cancers are an increasingly serious issue in many parts of the world. The aim of our study was to determine a possible relationship between Vitamin D receptor (VDR) gene polymorphisms and the risk of glioma and meningioma. Methods. We investigated the VDR Taq-I and VDR Fok-I gene polymorphisms in 100 brain cancer patients (including 44 meningioma cases a...

We describe a photochemical procedure for the sterilization of polynucleotides that are created by the Polymerase Chain Reaction (PCR). The procedure is based upon the blockage of Taq DNA polymerase when it encounters a photochemically modified base in a polynucleotide strand. We have discovered reagents that can be added to a PCR reaction mixture prior to amplification and tolerate the thermal...