background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

background: the emergence and spread of chloroquine resistant plasmodium falciparum in the world stimulated some investigators to consider different aspects of chloroquine resistance in human and rodent plasmodia . using animal plasmo­dia , particularly primate and rodent plasmodia can be useful model for human plasmodia studies. in this study we have tried to consider and compare the sequence ...

introduction: hematopoietic stem cells are always in a quiescence state. since they need retroviral transduction to infect dividing cells, they are resistant to retrovirus transduction. they need to be pre-stimulated by a cytokine cocktail. for proliferation without maturation, we suggest mip-1α as a novel factor. material and methods: retroviral vector produced by pg13/ln c8 cells titter on he...

human mitochondrial dna (mtdna) is 16,569 base pairs (bp) in length, coding for 37 genes. during the course of evolution, nearly all the genes expressing ribosomal proteins and ribosomal rnas (rrna) genes have been transferred from the mitochondria to the nucleus. however, mitochondrial dna contains two ribosomal rnas genes (12s and 16s), which have not yet transferred to the nucleus. these two...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...

chicken has been used as a suitable model for lipid metabolism studies, because dietary modifications especially dietary fat type can change chicken body composition. fats act as a condense source of energy and certain fatty acids such as polyunsaturated fatty acids (pufas) are required for both animal and human health. the n-3 pufas, especially, eicosapentaenoic acid (epa) and docosahexaenoic ...

thirtyfrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. polymorphism of cow lactoferrin (ltf) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). the results showed that lft gene pro...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study the polymorphism of the cast gene ofkhalkhali goat in azerbaijan province in iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (pcr-rflp). genomic dna was extracted from whole blood samples collected from 200 kh...

in this study the genetic polymorphism of growth hormone (gh) gene as a candidate gene in livestock was investigated. blood samples were randomly collected from 34 alpine and 42 saanen goats. dna was extracted from blood samples and a 365 bp region of exon 5 of the gh genewas amplified by polymerase chain reaction (pcr). pcr products were analyzed using single strand conformation polymorphism (...

background: many plant growth-promoting bacteria including rhizobia contain the 1-aminocyclopropane-1-carboxylate (acc) deaminase enzyme that can leave acc, and thereby lower the level of ethylene in stressed plants. drought and salinity are the most common environmental stress factors for plants in iran. objectives: the main aim of this research was development of bio-fertilizers containing ac...