Enabling technologies in synthetic biology now present the opportunity to engineer wine yeast for enhanced novel aromas. In doing so, improved products will increase desirability of consumer and add value winemaker. The action enzyme carotenoid cleavage dioxygenase 1 (CCD1) on ?-carotene produce ?-ionone is interest improve aroma flavour wine. Engineering yeast, Saccharomyces cerevisiae, higher...

introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...

BACKGROUND Ovarian yolk sac tumor (YST) is a very rare malignancy arising in young women. Chemotherapy has dramatically improved the prognosis. Current treatment consists of surgery followed by bleomycin, etoposide, and cisplatin (BEP) chemotherapy. However, given the rarity of this tumor, ovarian YST-specific survival and outcome after such treatment are not precisely known. PATIENTS AND MET...

Purpose More than 65,000 women are diagnosed with gynecologic cancers (i.e., ovarian, endometrial) in the United States each year. Exploratory laparotomy is a common primary treatment for these diagnoses; however, it leads to pain and distress in a majority of patients. Yoga is a promising intervention for improving these negative surgical outcomes. The purpose of this study was to explore the ...

Hydrogen sulfide (H2S) is the main volatile sulfur compound produced by Saccharomycescerevisiae during alcoholic fermentation and its overproduction leads to poor wine sensory profiles. Several factors modulate H2S production winemakers researchers require an easy quantitative tool quantify their impact. In this work, we developed a new sensitive method for evaluation of total using metal trap ...

Type III secretion systems harbored by several Gram-negative bacteria are often used to deliver host-modulating effectors into infected eukaryotic cells. About 20 core proteins are needed for assembly of a secretion apparatus. Several of these proteins are genetically and functionally conserved in type III secretion systems of bacteria associated with invertebrate or vertebrate hosts. In the Ys...

Phytohemagglutinin (PHA), the seed lectin of the common bean, accumulates in protein storage vacuoles of storage parenchyma cells in cotyledons. When expressed in yeast, PHA is efficiently targeted to the yeast vacuole [Tague and Chrispeels (1987). J. Cell Biol. 105, 1971-1979]. To identify vacuolar sorting information in PHA, a series of 3' deletions of the PHA gene were fused in-frame to a tr...

Cytokines, particularly interleukin (IL)-1beta, have been postulated to cause beta-cell destruction in type 1 diabetes. We tested the efficacy of an IL-1 cytokine trap in counteraction of suppressive and toxic effects after exposure of rat pancreatic islets in vitro to IL-1beta. The IL-1 cytokine trap used herein comprised extracellular domains of the IL-1 receptor accessory protein and the hum...