We have outlined a procedure that allows the large-scale screening of mutagenized Neurospora crassa populations for invertaseless mutants. We have isolated and characterized three mutations, inv(DBL1), inv(DBL9) and inv(DBL14), which have been mapped at or near the invertase structural gene. One of these, inv(DBL1), is particularly interesting. Our experiments indicate that the reduced level of...

An egg yolk-polymyxin medium (KG) for rapid enumeration of Bacillus cereus is described. The test is presumptive in that differentiation of B. cereus (and closely related organisms) from other species is based on the formation of turbidity in the agar surrounding the colonies of the cereus group organisms. The medium is formulated to encourage sporulation and release of free spores for serologi...

aflatoxin contamination of food and feedstuffs is a serious economic and health hazard. over a period of two years, 35 samples of raw pistachio nuts collected from different parts of iran were analysed for the presence of aflatoxigenic strains of the aspergillus flavus group. eighty-five isolates of a.flavus and a.parasiticuswere identified based on specific yello-orange pigment production in t...

The overall aim of this thesis was to develop, test and use a simple, clinically useful instrument for the nutritional screening of older patients. Four studies were performed, with a quantitative approach, in a geriatric rehabilitation ward in western Sweden. The number of patients who participated was: 56 (I), 114 (II), 147 (III) and 144 (IV) older patients. A nutritional screening instrument...

Effects of medium polarization are studied for 1S0 pairing in neutron and nuclear matter. The screening potential is calculated in the RPA limit, suitably renormalized to cure the low density mechanical instability of nuclear matter. The selfenergy corrections are consistently included resulting in a strong depletion of the Fermi surface. All medium effects are calculated based on the Brueckner...

Electrospray tandem mass spectrometry was applied to detect a series of inherited metabolic disorders during a newborn-screening pilot study and a selective screening in Japan. In our mass screening of 102,200 newborns, five patients with propionic acidemia, two with methylmalonic acidemia, two with medium-chain acyl-CoA dehydrogenase deficiency, three with citrullinemia type II, and one with p...

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious nosocomial and community-acquired infections, and accurate methods to detect such strains are needed. We tested the susceptibility of 3 kinds of MRSA isolation medium, MRSA Screen Agar, Oxacillin Resistance Screening Agar and CHROMagar MRSA. Both sensitivity and specificity of CHROMagar MRSA we...

background: acanthamoeba-related disease have a poor prognosis according to many previous studies. thus researches regarding biochemical and molecular aspects of this organism are a high priority. to this end achieving high amount of amoebae in culture is the first step for such studies. the main aim of the present research was to address the usage of tyi-s-33 (tripticase, yeast extract, iron-s...

Background: Chest X-ray is one of the examinations required for an annual health checkup. The interaction of radiation to the medium produces free radicals, which consequently causes biological changes either structural or properties of the cells. Whether the radiation from Chest X-ray upright technique affects the plasma membrane fluidity of thalassemic red blood cells (RBCs) is still unclear....