The production of alkaline protease by an Aspergillus flavus strain isolated in our laboratory by solid-substrate fermentation for use as a depilation agent and the influence of various factors on enzyme production are reported. The optimum conditions for maximum production were a growth temperature of 32 degrees C, 63% substrate moisture, and a growth period of 48 h. Enrichment with corn steep...

Saccharomyces cerevisiae in a medium without supplement of ergosterol. We now have isolated a new sterol from a culture broth of a Penicillium and found that this new sterol, named paxisterol, has analgesic activity in the mouse AcOH writhing assay1}. In contrast to corticosteroid, paxisterol does not have antiinflammatory activity. These results suggest that paxisterol is a new class of analge...

A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macrolide was isolated and identified as treponemycin (TP). The structure of the isolated compound was...

Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is able to produce a large amount of succinic acid in a medium containing glucose, peptone, and yeast extract. In order to reduce the cost of the medium, whey and corn steep liquor (CSL) were used as substrates for the production of succinic acid by M. succiniciproducens MBEL55E. Anaerobic batch cultures of M. succiniciproducens M...

We previously reported1} the isolation and structure determination of a novel anti-mite substance, AB3217-A (1), one of the components of an anti-mite substance complex produced by Streptomycesplatensis AB321 7. Following improvements in the isolation method of AB3217-Afrom cultured broth, we found that the strain produced two new minor components, AB3217-B (2) and C (3), which were structurall...

effect on T lymphocytes. Fermentations for the production of conagenin were done as follows. S. roseosporus was cultured in 500-ml Erlenmeyer flasks containing 1 10 ml of the autoclaved seed medium consisting of galactose 2.0%, dextrin 2.0%, soy peptone 1.0%, corn steep liquor 0.5%, (NH4)2SO4 0.2%, CaCO3 0.2% and one drop of silicone. The seed medium was adjusted to pH 7.4 with 1 n HC1 and was ...

Strains of Fusarium produced high levels of T-2 toxin when cultured on certain media absorbed into vermiculite. Modified Gregory medium was nutritionally complex (2% soya meal, 0.5% corn steep liquor, 10% glucose) and, when inoculated with the appropriate fungal strain, yielded maximum T-2 toxin within 24 days of incubation at 19 degrees C. On Vogel synthetic medium N (H. J. Vogel, Microb. Gen...

Pyralomicin 2a (5) -H -Cl -CH3 -CH3 Pyralomicin 2b (6) -H -CH3 -Cl -CH3 Pyralomicin2c(7) -H -Cl -CH3 -H Chemistry, Tokyo, Japan, produced new antibiotics, pyralomicins (1~7, Fig. 1). In this paper we report the production, isolation, physico-chemical properties and biological properties of 1 ~7. A slant culture of the pyralomicins-producing organism was inoculated into a 500-ml Erlenmyer flask ...

Anaerobic Digestion (AD) of wastewater for hydrogen production is a promising technology resulting in the generation value-added products and reduction organic load wastewater. The Two-Stage (TSAD) has several advantages over conventional single-stage process due to ability control acidification phase first bioreactor, preventing overloading and/or inhibition methanogenic population second bior...