background: interleukin-27 is a heterodimeric cytokine belonging to il-12 and il-23 families, secreted  by antigen presenting cells( apcs ) .the il-27 is composed of 2 subunits: epstein-barr virus induced gene 3 (ebi3) and p28. il-27 is an anti-inflammatory cytokine which has an inhibitory effect on th17 population and suppress the il-17 expression. it is suggested that il-27 could be a potent ...

We molecularly cloned the gag and env genes of the human immunodeficiency virus (HIV) and expressed fragments of these genes in Escherichia coli. Using the recombinant core and envelope proteins, we developed two competitive immunoassays (CIAs). Samples that recognized either the envelope or core proteins were considered positive for antibodies to HIV. This test system was comparable with weste...

The Western blot techniques that were originally established in the late 1970s are still actively utilized today. However, this traditional method of Western blotting has several drawbacks that include low quality resolution, spurious bands, decreased sensitivity, and poor protein integrity. Recent advances have drastically improved numerous aspects of the standard Western blot protocol to prod...

Recent recommendations for the serological diagnosis of Lyme disease include statements on quality assurance and the use of performance panels to assess laboratory competency. We used two performance panels--one from the Centers for Disease Control and Prevention (CDC) and one from Boston Biomedica Inc. (West Bridgewater, MA)-to evaluate the sensitivity and specificity of four western blot kits...

Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting sea...

The use of “Housekeeping proteins” as loading controls in western blots requires that they do not change their level of expression. However, deviations from this rule have been observed. This study investigated the expression of the commonly used proteins HSP60, -tubulin, -actin and GAPDH by western blot during retinoic acid-induced differentiation of the embryonal carcinoma NTera-2 and the neu...

The reaction of some antibodies with Western blots of protein shows strong non-specific binding especially at a region that corresponds to about 70-90 kDa. This binding is independent of protein concentration. Further analysis indicated that the factor responsible for the non-specific binding is 2-mercaptoethanol in the gel sample buffer. Gel electrophoresis of total tissue homogenates in the a...

Cryptosporidiosis is an acute diarrhoeal disease caused by Cryptosporidium spp for both human and animals. Typically, the duration of diarrhoeal illness and ultimate outcome of intestinal cryptosporidiosis depend on the immune status of the patient. Random serology-based studies in humans and animals have suggested that infection with this organism is common during a life time. 130 serum sample...

AIMS To identify antigens detected by western blotting in primary Toxoplasma gondii infection and determine their role in diagnosis of reactivated toxoplasmosis. METHODS Twenty three immunocompromised patients were tested by IgG western blotting. Patients were grouped retrospectively. Group 1 comprised 15 human immunodeficiency (HIV)/AIDS patients and included: group 1A (six patients with cli...