To explore the serum expression of long non-coding ribonucleic acid H19 and LOC554202 in cervical cancer as well clinical significance is main objective. Serum samples were collected from 52 preoperative patients, at same time, also 39 agematched women admitted due to gynecological benign diseases with screening negative selected control group. Among them, 10 patients used for postoperative com...

The human immunodeficiency virus type 1 (HIV-1) Tat protein enhances reverse transcription, but it is not known whether Tat acts directly on the reverse transcription complex or through indirect mechanisms. Since processing of Tat by HIV protease (PR) might mask its presence and, at least in part, explain this lack of data, we asked whether Tat can be cleaved by PR. We used a rabbit reticulocyt...

Retroviral reverse transcription is accomplished by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Here, we revealed an unprecedented role of 5'-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based on current consensus for HIV-1 transcription initiation site, HIV-1 transcripts possess a single G at 5'-ends (G1-form). However, we found...

To determine whether hepatitis C virus (HCV) replicates in bone marrow, we investigated positive- and negative-strand HCV RNA in bone marrow cells and fluids, and sera from patients with HCV infection. The study population consisted of 15 patients positive for antibodies to HCV (anti-HCV). Positive- and negative-strands HCV RNA were detected using highly strand-specific rTth reverse transcripti...

A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.

SURÝNEK JAKUB, VRTKOVÁ IRENA, KNOLL ALEŠ. 2016. Mycoplasma bovis Was not Detected in Milk from Dairy Cattle in the Czech Republic. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(1): 165–168. The aim of this study was to screen selected Czech dairy cattle farms for presence of Mycoplasma bovis. Between 2012 and 2014, 160 individual samples of cow milk, 60 colostrum sa...

Chrysanthemum chlorotic mottle viroid (CChMVd) is a serious pathogen infecting chrysanthemum worldwide. To improve and enhance the detection procedure, colorimetric loop-mediated isothermal amplifi cation (LAMP) technique was developed. Six LAMP primers were newly designed tested optimal conditions using recombinant plasmid of CChMVd as DNA template. The for incubation at 65°C 60 min. At these ...