The replication of herpes simplex virus type I and herpes simplex virus type 2 was studied in two isolated hamster cell lines. These lines developed after being treated with herpes simplex virus type 2 inactivated with 7,~2-dimethylbenz(a)anthracene. One cell line that developed, HDC-22, had a hypodiploid chromosome number and was missing a D-group chromosome. Growth studies revealed that this ...

Poliovirus replication has been studied in human lymphocytes during the course of blastogenesis under phytohemagglutinin (PHA) stimulation. Enhancement of virus replication in PHA-stimulated leukocyte cultures was due to an increase in number of virus-producing cells. Virus yield was approximately 10 plaque-forming units (PFU) per producing cell, both in stimulated and in nonstimulated cultures...

Herpes simplex virus type 1 (HSV)-host cell interactions were studied in fibroblasts from inbred mice by measuring virus replication, virus adsorption, infectious center formation, and single-cell virus production. BALB/c mouse embryo fibroblasts (MEF) produced more intracellular and extracellular virus than C57BL/6 MEF, with differences in virus production first appearing at 16 hr after inocul...

Several viruses, including influenza, induce an imbalance of intracellular redox state toward pro-oxidant conditions. Through different mechanisms these alterations contribute both to influenza virus replication and to the pathogenesis of virus-induced disease. At the same time, influenza virus activates several intracellular signaling pathways involved in important physiological functions of t...

Vaccinia virus and African swine fever virus are deoxyribonucleic acid viruses of cytoplasmic origin. The fact that rifampin inhibits the replication of the former virus led to an investigation of its effect on African swine fever virus. The virus used was cytopathogenic to a PK-15 cell line, hemadsorbing in pig leukocyte cultures and lethal to pigs. Rifampin clearly inhibited the multiplicatio...

BACKGROUND Influenza A virus (IVA) exploits diverse cellular gene products to support its replication in the host. The significance of the regulatory (beta) subunit of casein kinase 2 (CK2beta) in various cellular mechanisms is well established, but less is known about its potential role in IVA replication. We studied the role of CK2beta in IVA-infected A549 human epithelial lung cells. RESUL...

The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantifi...

Cyclophilin A (CypA) is the main member of the immunophilin superfamily that has peptidyl-prolyl cis-trans isomerase activity. CypA participates in protein folding, cell signaling, inflammation and tumorigenesis. Further, CypA plays critical roles in the replication of several viruses. Upon influenza virus infection, CypA inhibits viral replication by interacting with the M1 protein. In additio...

Replication of positive-strand RNA virus is mediated by a virus- encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated...