Amidation is the predominant reaction within pharmaceutical setting, and it attracting greater attention due to increased demand for therapeutic peptides. The high efficacy safety profile of peptides have placed these molecules in prime position arena, which reflected by receiving several approvals from various regulatory agencies each year. In this context, developing efficient strategies pept...

Esterolytic activity of three enzymic cholesterol reagents was evaluated with use of human serum-based reference materials. Primary cholesterol standards were used to calibrate the enzymic methods, and incomplete cholesteryl ester hydrolysis was measured as the bias between the enzymic cholesterol values and corresponding reference values. The average biases observed with eight reference specim...

The sensitivity, specificity, positive and negative predictive values of a reagent strip test for the diagnosis of schistosomiasis mansoni by detecting circulating cathodic antigen (CCA) in urine were evaluated using 184 stool and urine samples collected from schoolchildren living in relatively low endemic area of schistosomiasis mansoni in Ethiopia. A combined result of stool samples processed...

The paper discusses two procedures of flotation of difficult-to-float oxidized coal. The first one is the normal flotation procedure which relies on wetting coal with water followed by addition of flotation reagents (dodecane, dodecyl tetraoxyethelene ether (C12E4), and 1-pentanol). The second, direct contact procedure consists of mixing pure reagents with dry coal followed by addition of water...

An convenient reagent for the one-carbon homologation of an aldehyde to the corresponding alkyne is reported. This reagent allows this conversion to conveniently be carried out on a large scale under ambient conditions.

Measles is an acute febrile rashes and infections caused by the measles virus belonging to the paramyxovirus. Each year more than twenty million peoples are affected to measles all over the world and 242,000 patients are leading to death. Traditionally the diagnosis of measles had been based on clinical symptoms in daily clinical practice. In recent years, many clinical cases do not show typica...

BACKGROUND Good laboratory practice includes verifying that each new lot of reagents is suitable for use before it is put into service. Noncommutability of quality control (QC) samples with clinical patient samples may preclude their use to verify consistency of results for patient samples between different reagent lots. METHODS Patient sample results and QC data were obtained from reagent lo...

Methylenedioxymethamphetamine (MDMA) is structurally related to methamphetamine (MA). There are many different commercially available immunoassay (IA) reagents for the initial screening of amphetamine and/or methamphetamine. These reagents may be employed to detect MDA/MDMA in urine samples. In order to select a suitable reagent for the initial screening of MDMA in urine samples, we evaluated 7...

N-Bromoacetylethanolamine phosphate, prepared by the bromoacetylation of ethanolamine phosphate, has been tested as an active site-specilic reagent for rabbit and rat muscle fructose diphosphate aldolases. The reagent inactivates both enzymes, and inactivation is prevented by substrates or competitive inhibitors. Loss of activity is pseudo-first order until the later stages of inactivation, and...

The use of Fenton's reagent (Fe2+/H2O2) and Fenton-like reagents containing transition metals of Cu(II), Zn(II), Co(II), and Mn(II) for an alum sludge conditioning to improve its dewaterability was investigated. The results obtained were compared with those obtained from conditioning the same alum sludge using cationic and anionic polymers. Experimental results show that Fenton's reagent was th...