The relationships of lipoprotein cholesterol and triglyceride among lipoprotein fractions have potential significance for understanding atherogenesis and distinguishing among different classes of hyperlipidemia. We have compared these relationships in normolipidemic, hypercholesterolemic, hypertriglyceridemic, and combined hyperlipidemic participants in the Pacific Northwest Bell Telephone Comp...

Plasma phospholipid transfer protein (PLTP) is thought to be involved in the remodeling of high density lipoproteins (HDL), which are atheroprotective. It is also involved in the metabolism of very low density lipoproteins (VLDL). Hence, PLTP is thought to be an important factor in lipoprotein metabolism and the development of atherosclerosis. We have overexpressed PLTP in mice heterozygous for...

Eleven hybridoma antibodies specific for apoprotein B were used to examine the expression of apoprotein B epitopes on native plasma lipoproteins using fluid phase radioimmunoassays. Heterogeneity of apoprotein B in low density lipoprotein (LDL) of density 1.019-1.063 g/ml was indicated by maximum binding of radioiodinated LDL in the presence of excess antibody that ranged from 45 to 100%. Affin...

During the period 1971 to 1975, the cholesterol content of lipoprotein density classes were measured in 2415 male and female Framingham Heart Study participants and 4342 of their offspring and offspring spouses who were free of coronary heart disease. In this first of three papers, the univariate and bivariate distributions of high density lipoprotein cholesterol, low density lipoprotein choles...

The effect of heparin on the activity of lipoprotein lipase from bovine milk was investigated under conditions varying with regard to ionic strength, purity of enzyme, and mode of substrate activation. A crude enzyme (dialyzed skim milk) was studied, along with a purified preparation obtained by affinity chromatography on a column of heparin-substituted agarose. Serum, high density lipoproteins...

A method has been described for the measurement of apoB concentration and specific activity in very low density lipoprotein (VLDL) and low density lipoprotein (LDL) during metabolic studies. For measurement of specific activity, apoB was separated from other apolipoproteins and lipids by precipitation in, and subsequent washing with, isopropanol. For determination of apoB concentration, equal v...

We evaluated the density-gradient ultracentrifugation method in a swinging-bucket rotor (Anal Biochem 111, 149-157, 1981) in a slightly modified version for isolation and determination of high-density lipoprotein (HDL) subfractions. We prestained the serum with Coomassie Brilliant Blue R, which did not change the hydrated densities of the lipoproteins, and after only 2.2 X 10(8) gav . min obtai...

A prospective study of autopsy-determined measures of atherosclerosis in aortas and coronary and cerebral arteries was completed for a group of 83 men who were free of clinical cardiovascular disease and cancer when they entered the Cooperative Lipoprotein Phenotyping Study in 1970. Total cholesterol minus high density lipoprotein cholesterol (HDL-C) was significantly associated with measures o...

nonenzymatic glycation of low density lipoprotein (ldl) is a reaction of glucose and other reducing sugars with apolipoprotein b100 (apo-b100) lysine residues. in diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. the objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on ldl glycatio...

osteonectin is a bone- associated protein involved in vascular calcification. adiponectin may protect against cardiovascular disease but possible effects on vascular calcification have been poorly studied. the aim of this study was to investigate the modulatory effect of adiponectin on oxidized low density lipoprotein (oxldl)- induced expression of osteonectin in human aorta vascular smooth mus...