Among uropathogens, uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infection (UTI) worldwide, but clinical aspects due to this bacterial species is not fully understood in southern Thailand. Two hundred fifty-four UPEC isolates from patients admitted to Maharaj Nakhon Si Thammarat Hospital, southern Thailand were examined for crucial virulence genes, showing tha...

Introduction: Uropathogenic Escherichia coli (UPEC) is among the primary cause of urinary tract infections (UTIs). This study was conducted to determine the antibiotic resistance pattern of E. coli isolates from UTIs in Kermanshah. Methods: In this study, 180 midstream urine samples were collected from outpatients for five months. Based on differential biochemical tests such as oxidase, Indole,...

Background and Aims: Uropathogenic Escherichia coli (UPEC) are considered major reservoir for genes encoding antimicrobial resistance. The mechanism of resistance and persistence of UPEC has been attributed to the production of biofilm and Extended Beta Lactamase (ESBL). This hospital-based prospective study determined how biofilm and ESBL production facilitate antibacterial resistance amongst ...

The molecular initiators of infection-associated pain are not understood. We recently found that uropathogenic E. coli (UPEC) elicited acute pelvic pain in murine urinary tract infection (UTI). UTI pain was due to E. coli lipopolysaccharide (LPS) and its receptor, TLR4, but pain was not correlated with inflammation. LPS is known to drive inflammation by interactions between the acylated lipid A...

A total of 179 urine samples were collected from patients suffering urinary tract infections admitted and visit Al-Hilla General Teaching Hospital in City, during a period April 2021 to December 2021, both sex (male female). Out 179,123 (68.7%) positive culture, whereas 56 (31.3%) showed no bacterial growth, To confirm the identification E. coli by use selective media (EMB agar medium, biochemi...

Objective: Integrons, which are highly effective in capturing, integrating and expressing gene cassettes, play an important role the dissemination of multiple antibiotic resistances. This study investigated correlations integrons uropathogenic E. coli (UPEC) with resistance, virulence phylogeny also relationships phylogroups resistance. Material Methods: Fifty UPECs isolated from uncomplicated ...

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previously reported marked FimH mutants; these are now improved versions useful for definitive assignmen...

Abstract Uropathogenic Escherichia coli (UPEC) is a causative agent of urinary tract infections (UTIs), and one the most common causes pyelonephritis. Interestingly, UPEC often found as non-pathobiont in gut flora. The transition from flora to pathogen for dependent upon flexible tropism, number virulence factors that aid UPEC’s colonization invasion tract. We recently demonstrated role E. aryl...

Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class 1 integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli w...

The antibacterial defense against infections depends on the cooperation between distinct phagocytes of the innate immune system, namely macrophages and neutrophils. However, the mechanisms driving this cooperation are incompletely understood. In this study we describe the crosstalk between Ly6C⁺ and Ly6C(-) macrophage-subtypes and neutrophils in the context of urinary tract infection (UTI) with...