A membrane filter procedure for enumerating Escherichia coli was developed and evaluated. The method quantifies E. coli within 24 h without requiring subculture and identification of isolates. It incorporates a primary selective-differential medium for gram-negative, lactose-fermenting bacteria; resuscitation of weakened organisms by incubation for 2 h at 35 degrees C before incubation at 44.5 ...

INTRODUCTION Acinetobacter baumannii and Brucella species are Gram-negative organisms that are vulnerable to misinterpretation as Gram-positive or Gram-variable in blood cultures. OBJECTIVE We assess the random errors in gram stain interpretation to reduce the likelihood of such errors and therefore patient harm. METHODOLOGY Aerobic and anaerobic blood cultures from two patients in an acute...

To infect a susceptible host, the gastrointestinal pathogen Yersinia enterocolitica must survive passage through the acid environment of the stomach. In this study, we showed that Y. enterocolitica serotype O8 survives buffered acidic conditions as low as pH 1.5 for long periods of time provided urea is available. Acid tolerance required an unusual cytoplasmically located urease that was activa...

A new facultative alkaliphilic Bacillus species isolated from mica mines of Nellore district of Andhra Pradesh, India. This strain is gram positive, rod shaped, motile bacteria capable of growth in aerobic conditions up to pH 12.0 exhibiting optimum growth at pH 10.0. The strain is oxidative, catalase positive, urease positive, able to hydrolyse starch, gelatine, able to utilize lactose, Dgluco...

BACKGROUND The gastric biopsy urease test is the most frequently used test for the diagnosis of Helicobacter pylori infection in routine gastrointestinal endoscopy practice. In Malaysia up to recently, only one commercial biopsy urease test was available: the CLO test (Ballard Medical Products, Draper, Utah, USA). Large endoscopy units use their own 'homemade' unbuffered ultra rapid urease test...

Microbial-induced calcite precipitation (MICP) is a relatively green and sustainable soil improvement technique. It utilizes biochemical process that exists naturally in soil to improve engineering properties of soils. The calcite precipitation process is uplifted by the mean of injecting higher concentration of urease positive bacteria and reagents into the soil. The main objective of this pap...

Gut-associated microbiota of ants include Rhizobiales bacteria with affiliation to the genus Bartonella. These bacteria may enable the ants to fix atmospheric nitrogen, but no genomes have been sequenced yet to test the hypothesis. Sequence reads from a member of the Rhizobiales were identified in the data collected in a genome project of the ant Harpegnathos saltator. We present an analysis of...

Ureolytic bacteria are key organisms in the rumen producing urease enzymes to catalyze the breakdown of urea to ammonia for the synthesis of microbial protein. However, little is known about the diversity and distribution of rumen ureolytic microorganisms. The urease gene (ureC) has been the target gene of choice for analysis of the urea-degrading microorganisms in various environments. In this...

The search for alternative methods treating and preventing bee dysbacteriosis is a priority beekeeping as branch of veterinary medicine. Lime honey, buckwheat flower forest acacia honey were tested to evaluate their antagonistic effect against test culture enterobacteria bees Klebsiella pneumoniae species. study was conducted in several stages: 1. Determine the activity microflora pure pathogen...