BACKGROUND Controlled generation and removal of hydrogen peroxide play important roles in cellular redox homeostasis and signaling. We used a hydrogen peroxide biosensor HyPer, targeted to different compartments, to examine these processes in mammalian cells. PRINCIPAL FINDINGS Reversible responses were observed to various redox perturbations and signaling events. HyPer expressed in HEK 293 c...

This in vivo study determined the kinetics of 3% hydrogen peroxide in a bleaching gel within the first hour. The material used in this study was 3% hydrogen peroxide gel (Perfecta 3/15, Premier Dental Products Co) and the study involved 10 subjects who met the inclusion and exclusion criteria. Each subject wore the tray with gel six different times on separate days. Evaluation of the remaining ...

A range of some Iranian monofloral honeys were assayed for antibacterial activity with and without hydrogen peroxide which is inactivated by the addition of catalase. I t was found that the high amount of antibacterial activity in honeys was due to a factor other than hydrogen peroxide. The test microorganism Staphylococcus aureus, was not inhibited by the acidity or the osmolarity of the h...

Hydrogen peroxide induces oxidation and consequently inactivation of many protein tyrosine phosphatases. It was found that hydrogen peroxide, in the presence of carboxylic acids, was efficiently activated to form even more potent oxidant - peroxy acid. We have found that peroxytetradecanoic acid decreases the enzymatic activity of CD45 phosphatase significantly more than hydrogen peroxide. Our ...

A feasible and fast method to fabricate hydrogen peroxide sensor was investigated by graphene-manganase nanocomposite carbone paste electrode. In the present work, in first step, the graphene was synthesized by chemical method and in second step, manganese oxide nanoparticle was doped on graphene. graphene-manganase nanocomposite was characterized by FTIR and SEM. The nanocomposite shows a high...

AIM To measure the time course hydrogen peroxide penetration into the pulp cavity and evaluate short-term tooth color changes after bleaching. MATERIALS AND METHODS Twenty extracted human canines were sectioned, pulp tissue removed and the cavity enlarged. Teeth were painted with nail varnish to leave a 6-mm diameter circle on the buccal surface. Baseline color was measured spectrophotometric...

Hydrogen peroxide sensing microelectrodes for scanning electrochemical microscopy (SECM) have been developed and used to measure hydrogen peroxide in the diffusion layer during electrochemical reduction of oxygen on gold and carbon electrodes. These microelectrode biosensors were also used to detect immobilized glucose oxidase through the production of hydrogen peroxide during enzymatic oxidati...

Hydrogen peroxide is one of the fundamental molecules of biology, regulating key cell signaling pathways and the development of numerous inflammatory diseases. There is therefore great interest in developing contrast agents that can detect hydrogen peroxide in vitro and in vivo. In this report, we present a new contrast agent for imaging hydrogen peroxide, termed the chemiluminescent poly(ethyl...