This document lists citations for peer-reviewed journal articles that support a physiological basis for MCS. Disruption of the blood-brain barrier and neuronal cell death in cingulate cortex, dentate gyrus, thalamus, and hypothalamus in a rat model of Gulf-War syndrome. combined exposure to low doses of pyridostigmine bromide, DEET, and permethrin produce neurochemical and neuropathological alt...

The muscularis externa of the intestinal wall of frogs was fixed in osmium tetroxide, embedded in Vestopal-W, serially sectioned for electron microscopy, and stained with uranyl acetate. A method to obtain individually mounted and properly positioned serial sections is described. The three-dimensional techniques used during the course of this investigation demonstrate that it is possible to exa...

Stalks and loricae of chrysomonads were studied by fluorescence microscopy employing Calcoflor White-Evans blue (CW-Eb) staining, and by uranyl acetate staining of dried, whole mount preparations for electron microscopy. These structures were composed of microfibrils approximately 4 nm in diameter embedded in a matrix. The organization of the loricae of Poterioochromonas malhamenesis, "Amimonas...

PtK2 cells were grown on gold grids and treated with Triton X-100 in a microtubule stabilizing buffer. The resulting cytoskeletons were fixed with glutaraldehyde and subjected to the indirect immunofluorescence procedure using monospecific tubulin antibodies. Grids were examined first by fluorescence microscopy, and the display of fluorescent cytoplasmic microtubules was recorded. The grids wer...

We present methods for embedding brain tissue in Lowicryl K4M embedding medium and localizing antigens using postembedding immunogold techniques. After perfusion fixation with 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M cacodylate buffer, blocks of rat brain were placed in 2% aqueous uranyl acetate for 1 hour, dehydrated in 50%, 70%, and 95% ethanol, infiltrated with Lowicryl/ethanol m...

Two kinds of droplets are revealed by the electron microscope in the milk, secretory alveoli, and epithelial cells of the lactating mammary gland of rats and mice (1-3). The larger droplets are fat and the smaller ones, measuring 30 to 400 m# in diameter, are primarily protein (4). The protein droplets first appear enclosed in vacuoles in the Golgi region; these vacuoles presumably pass through...

the main goal of this study is to investigate the capability of zeolites a and p synthesized from iranian natural clinoptilolite for uranium uptak. the removal of uranium(vi) from aqueous solution via ion exchange by zeolites in a single component system with various contact times,  temperatures and initial concentrations of uranium(vi) was investigated. the experimental results were fitted to ...

Pieces of both unfixed and fixed rat adrenal cortex were frozen and cut with a cryoultramicrotome. In unstained frozen sections of unfixed adrenals patches of heterochromatin were seen in the nucleus, while numerous round uniformly dense structures, the mitochondria, could be identified in the cytoplasm. Lipid droplets appear as small ligh round areas. Staining the sections with potassium perma...

Buckley, Patricia M. (University of California, Davis), Virginia E. Sjaholm, and N. F. Sommer. Electron microscopy of Botrytis cinerea conidia. J. Bacteriol. 91:2037-2044. 1966.-Sections of germinating and nongerminating Botrytis cinerea conidia were examined with an electron microscope. Uranyl acetate or lead citrate provided contrast between membranes and cytoplasm. Membrane-bounded, dense in...

The feasibility of using the Hale stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Three kinds of mouse ascites tumor cells were fixed in neutral buffered formalin, exposed to fresh colloidal ferric oxide, treated with potassium ferrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Additional staining with uranyl acetate and...