Osmanthus fragrans (Thunb.) Lour. flowers are important natural resource of phenolic compounds, which have antioxidant activities and been consumed as functional food dietary supplements for thousand years. Ultra-performance liquid chromatography coupled with electrochemical detection (UPLC-ECD) was developed to simultaneously analyze the eight compounds in collected from different regions acro...

Major royal jelly proteins (MRJPs) have been used as endogenous biomarkers for honey authentication. However, no study on quantification of MRJPs in authentication was reported. In order to develop a new authenticity method based quantitative analysis honey, trypsin-digested peptides from were examined by mass spectrometry (MS). Three peptides, YNGVPSSLNVISK, TLQMIAGMK, and LTVAGESFTVK selected...

A fixed-dose combination of paracetamol (PCM) and caffeine (CAF) tablets/capsules is the most frequently used over-the-counter medicine for fever headache. In this paper, a simple, reliable, sensitive, rapid, stability-indicating ultra-performance liquid chromatography (UPLC) analytical method was proposed simultaneously assessing PCM CAF in pharmaceutical formulations. The UPLC developed on an...

Bilastine, is a second-generation antihistamine drug which used in the treatment of allergic rhinoconjunctivitis and urticaria. It exerts its effect as selective histamine H1 receptor antagonist. In this study, an ultra-high-performance liquid chromatography (UPLC) method was developed to measure concentrations Bilastine mouse blood, applied measuring pharmacokinetics analyte after oral intrave...

Valnemulin (VML) is a semi-synthetic pleuromutilin derivative widely used to treat animal bacterial diseases. However, no study has comprehensively evaluated VML metabolism in aquatic animals, including crucian carp. This aimed investigate metabolites carp were quantified via intraperitoneal injection and analyzed ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Th...

• Green extraction of phenolic compounds from the cocoa shell was modeled and optimized. Response surface methodology artificial neural networks showed comparable estimation abilities. Spectrophotometric results correlate with UPLC-measured individual compounds. Major phenolics in were protocatechuic acid mono- dimeric flavanols. An eco-friendly method using just water is presented to revaloriz...

A Novel UPLC–MS/MS Method for Determination of γ-Amino Butyric acid Analogue in Human Plasma: Application to Pharmacokinetic StudyAnju Aji, Sarita Karthikeyan, Sarabjit Singh, Shivanand P. Puthli

This study employed the response surface methodology to optimize extraction conditions for recovering vitamins D2 and K1 from green leafy vegetables using ultrasonication-assisted extraction. The vitamin content was determined an Accucore C18 column a UPLC-Q-ToF/MS method. An RSM-I-Optimal design used designing experiment find best combination of solvent level (mL), sonication time (min), frequ...