To study the genetic control of plant responses to cold stress, Arabidopsis thaliana mutants were isolated by a screen for mutations that impair cold-induced transcription of the CBF3-LUC reporter gene. We report here the characterization and cloning of a mutated gene, atnup160-1, which causes reduced CBF3-LUC induction under cold stress. atnup160-1 mutant plants display altered cold-responsive...

The regulation of ICE1 protein stability is important to ensure effective cold stress response, and is extensively studied in Arabidopsis. Currently, how ICE1 stability in fruits under cold stress is controlled remains largely unknown. Here, we reported the possible involvement of a SEVEN IN ABSENTIA (SINA) ubiquitin ligase MaSINA1 from banana fruit in affecting MaICE1 stability. MaSINA1 was id...

Protein aggregation is a long-standing problem for preservation of proteins in both laboratory settings and for commercial biotechnology products. It is well established that heating (cooling) can accelerate (slow) aggregation by populating (depopulating) unfolded or partially unfolded monomer states that are key intermediates in aggregation processes. However, there is a long-standing question...

using cold working fastener holes is a simple life enhancement procedure that strengthens metallic components by retarding crack growth around a hole. in the present paper, the residual stress field that is induced by the hole expansion process is obtained based on different material models namely elastic perfect plastic, isotropic hardening and kinematic hardening using three-dimensional finit...

BACKGROUND AND AIMS Polyphenol oxidases (PPOs) catalyse the oxidation of monophenols and/or o-diphenols to highly reactive o-quinones, which in turn interact with oxygen and proteins to form reactive oxygen species (ROS) and typical brown-pigmented complexes. Hence PPOs can affect local levels of oxygen and ROS. Although the currently known substrates are located in the vacuole, the enzyme is t...

Cold is an environmental factor limiting the growth and yield of agricultural crops. To find cold-tolerant pistachio rootstocks, a factorial experiment was conducted at Pistachio Research Center (PRC), Rafsanjan, Iran during 2017-2018. The factors included six rootstocks (Badami Zarand, Ghazvini, Sarakhs, Integerrima × Badami Zarand, Integerrima × Ghazvini and Integerrima × Sarakhs) and four th...

The spotted wing Drosophila, Drosophila suzukii, is a new pest in Europe and America which causes severe damages, mostly to stone fruit crops. Temperature and humidity are among the most important abiotic factors governing insect development and fitness. In many situations, temperature can become stressful thus compromising survival. The ability to cope with thermal stress depends on basal leve...

Low temperature induces the expression of many plant genes through undefined signaling pathways. To gain insight into cold signal transduction mechanisms, we isolated Arabidopsis mutants that exhibited altered regulation of low temperature-induced gene expression. One such mutant, hos2, was shown previously to have an enhanced induction of stress-responsive genes by cold, whereas the expression...